{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:18:14Z","timestamp":1785421094116,"version":"3.56.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Societe de Pathologie Infectieuse de Langue Francaise"},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI173803"],"award-info":[{"award-number":["R01AI173803"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,8,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Toxoplasma gondii<\/jats:italic>\n                      is an obligate intracellular protozoan parasite that can establish lifelong infections and cause severe disease in immunocompromised individuals. Interferon gamma (IFN\u03b3) is a key host defense cytokine that induces a variety of toxoplasmacidal mechanisms. Recent CRISPR\/Cas9 loss-of-function screens identified multiple\n                      <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n                      genes important for fitness in IFN\u03b3-stimulated cells. One consistent hit in several screens was the parasite surface antigen, SAG1. Here, we used CRISPR\/Cas9 to generate a\n                      <jats:italic toggle=\"yes\">SAG1<\/jats:italic>\n                      knockout strain and found that SAG1 is important for parasite fitness specifically in IFN\u03b3-stimulated cells. Mechanistic studies revealed that host surface sialic acids are important for parasite attachment, especially in IFN\u03b3-stimulated cells. SAG1-deficient parasites had reduced attachment efficiency, which was exacerbated in IFN\u03b3-treated cells. These findings highlight the role of SAG1 in mediating robust parasite attachment, especially in the context of immune pressure.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00010-25","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T10:15:36Z","timestamp":1751537736000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Surface antigen SAG1 mediates\n                    <i>Toxoplasma gondii<\/i>\n                    fitness and host cell attachment in IFN\u03b3-stimulated cells"],"prefix":"10.1128","volume":"93","author":[{"given":"Olivia","family":"Cohen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rrcem69","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis","place":["Davis, USA"]},{"name":"Service de Maladies Infectieuses et Tropicales, CHU Toulouse","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parag","family":"Maru","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rrcem69","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinli","family":"Liang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rrcem69","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0289-7109","authenticated-orcid":true,"given":"Jeroen P. 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