{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T10:21:55Z","timestamp":1793701315730,"version":"4.5.1"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T00:00:00Z","timestamp":1529539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Wellcome Trust Senior Clinical Research Fellowship","award":["095780\/Z\/11\/Z"],"award-info":[{"award-number":["095780\/Z\/11\/Z"]}]},{"name":"FP7 NEWTBAC","award":["241745"],"award-info":[{"award-number":["241745"]}]},{"name":"European Union Horizon 2020 research and Innovations programme","award":["643381"],"award-info":[{"award-number":["643381"]}]},{"name":"National Institutes of Allergy and Infectious diseases, partly funded the antigens and peptide pools provided by Lewinsohn D. laboratory","award":["HHSN272200900053C"],"award-info":[{"award-number":["HHSN272200900053C"]}]},{"name":"National Institutes of Health, partly funded the antigens and peptides pools provided by Lewinsohn D laboratory","award":["HHSN266200400081C"],"award-info":[{"award-number":["HHSN266200400081C"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The development of a vaccine against tuberculosis (TB), a disease caused by\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    , is urgently needed. The only currently available vaccine,\n                    <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n                    BCG, has variable efficacy. One approach in the global vaccine development effort is focused on boosting BCG using subunit vaccines. The identification of novel antigens for inclusion in subunit vaccines is a critical step in the TB vaccine development pathway. We selected four novel mycobacterial antigens recognized during the course of human infection. A replication-deficient chimpanzee adenovirus (ChAdOx1) was constructed to express each antigen individually, and these vectors were evaluated for protective efficacy in murine\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    challenge experiments. One antigen, PPE15 (Rv1039c), conferred significant and reproducible protection when administered alone and as a boost to BCG vaccination. We identified immunodominant epitopes to define the protective immune responses using tetramers and intravascular staining. Lung parenchymal CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    CXCR3\n                    <jats:sup>+<\/jats:sup>\n                    KLRG1\n                    <jats:sup>\u2212<\/jats:sup>\n                    T cells, previously associated with protection against\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    , were enriched in the vaccinated groups compared to the control groups. Further work to evaluate the protective efficacy of PPE15 in more stringent preclinical animal models, together with the identification of further novel protective antigens using this selection strategy, is now merited.\n                  <\/jats:p>","DOI":"10.1128\/iai.00014-18","type":"journal-article","created":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T10:14:06Z","timestamp":1523528046000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["Identification and Evaluation of Novel Protective Antigens for the Development of a Candidate Tuberculosis Subunit Vaccine"],"prefix":"10.1128","volume":"86","author":[{"given":"Elena","family":"Stylianou","sequence":"first","affiliation":[{"name":"The Jenner Institute, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Harrington-Kandt","sequence":"additional","affiliation":[{"name":"The Jenner Institute, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Beglov","sequence":"additional","affiliation":[{"name":"The Jenner Institute, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Bull","sequence":"additional","affiliation":[{"name":"The Jenner Institute, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawamin","family":"Pinpathomrat","sequence":"additional","affiliation":[{"name":"The Jenner Institute, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendolyn M.","family":"Swarbrick","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Oregon Health &amp; Sciences University, Portland, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah A.","family":"Lewinsohn","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Oregon Health &amp; Sciences University, Portland, Oregon, USA"},{"name":"Department of Molecular Microbiology and Immunology, Oregon Health &amp; Sciences University, Portland, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Lewinsohn","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Oregon Health &amp; Sciences University, Portland, Oregon, USA"},{"name":"Department of Medicine, Oregon Health &amp; Sciences University, Portland, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"McShane","sequence":"additional","affiliation":[{"name":"The Jenner Institute, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Global tuberculosis report","author":"World Health Organization","year":"2015","unstructured":"World Health Organization. 2015. 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