{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T09:16:38Z","timestamp":1784193398188,"version":"3.55.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Haemophilus ducreyi<\/jats:named-content>\n            , the etiologic agent of chancroid, has an obligate requirement for heme. Heme is acquired by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. ducreyi<\/jats:named-content>\n            from its human host via TonB-dependent transporters expressed at its bacterial surface. Of 3 TonB-dependent transporters encoded in the genome of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. ducreyi<\/jats:named-content>\n            , only the hemoglobin receptor, HgbA, is required to establish infection during the early stages of the experimental human model of chancroid. Active immunization with a native preparation of HgbA (nHgbA) confers complete protection in the experimental swine model of chancroid, using either Freund's or monophosphoryl lipid A as adjuvants. To determine if transfer of anti-nHgbA serum is sufficient to confer protection, a passive immunization experiment using pooled nHgbA antiserum was conducted in the experimental swine model of chancroid. Pigs receiving this pooled nHgbA antiserum were protected from a homologous, but not a heterologous, challenge. Passively transferred polyclonal antibodies elicited to nHgbA bound the surface of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. ducreyi<\/jats:named-content>\n            and partially blocked hemoglobin binding by nHgbA, but were not bactericidal. Taken together, these data suggest that the humoral immune response to the HgbA vaccine is protective against an\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. ducreyi<\/jats:named-content>\n            infection, possibly by preventing acquisition of the essential nutrient heme.\n          <\/jats:p>","DOI":"10.1128\/iai.00017-11","type":"journal-article","created":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T04:40:50Z","timestamp":1307421650000},"page":"3168-3177","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Passive Immunization with a Polyclonal Antiserum to the Hemoglobin Receptor of Haemophilus ducreyi Confers Protection against a Homologous Challenge in the Experimental Swine Model of Chancroid"],"prefix":"10.1128","volume":"79","author":[{"given":"Isabelle","family":"Leduc","sequence":"first","affiliation":[{"name":"Departments of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William G.","family":"Fusco","sequence":"additional","affiliation":[{"name":"Departments of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelima","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Departments of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patty A.","family":"Routh","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah M.","family":"Cholon","sequence":"additional","affiliation":[{"name":"Departments of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia M.","family":"Hobbs","sequence":"additional","affiliation":[{"name":"Departments of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"},{"name":"Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glen W.","family":"Almond","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Orndorff","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Elkins","sequence":"additional","affiliation":[{"name":"Departments of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"},{"name":"Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2224-2232.2006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2007.02843.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/315309"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/314483"},{"key":"e_1_3_2_6_2","first-page":"126","article-title":"Study of genital lesions","volume":"69","author":"Anand Kumar B. H.","year":"2003","unstructured":"Anand KumarB. H.VijayaD.RaviR.ReddyR. R.. 2003. Study of genital lesions. Indian J. Dermatol. Venereol. Leprol. 69:126\u2013128.","journal-title":"Indian J. Dermatol. Venereol. Leprol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.3.2.295"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.9.4290-4298.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181bd1007"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)01639-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/516471"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.4.1241-1245.1995"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1608-1619.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00217-10"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.13.4.608"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181bf5a98"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.5.1764-1773.1997"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-65502-9_1"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/160.3.380"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/586901"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00994-07"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-34-6-317"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.79.1.68"},{"key":"e_1_3_2_24_2","first-page":"83","article-title":"Agents of the \u201csuis-ide diseases\u201d of swine: Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis","volume":"63","author":"MacInnes J. I.","year":"1999","unstructured":"MacInnesJ. I.DesrosiersR.. 1999. Agents of the \u201csuis-ide diseases\u201d of swine: Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis. Can. J. Vet. Res. 63:83\u201389.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_2_25_2","first-page":"98","article-title":"Cutaneous chancroid in a visitor from Vanuatu. Australas","volume":"49","author":"McBride W. J.","year":"2008","unstructured":"McBrideW. J.HannahR. C.Le CornecG. M.BletchlyC.. 2008. Cutaneous chancroid in a visitor from Vanuatu. Australas. J. Dermatol. 49:98\u201399.","journal-title":"J. Dermatol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1086\/515664"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00034-09"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134247"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3694-3702.2002"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1086\/496979"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.2010.tb03537.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.041814"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.2.233"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600830"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01016-06"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5345-5351.1999"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0340025"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.4.1346-1351.1993"},{"key":"e_1_3_2_39_2","first-page":"818","article-title":"Eradicating chancroid","volume":"79","author":"Steen R.","year":"2001","unstructured":"SteenR. 2001. Eradicating chancroid. Bull. World Health Organ. 79:818\u2013826.","journal-title":"Bull. World Health Organ."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.5.1724-1735.1996"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.034207"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.9.4254-4262.1998"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4438-4446.2001"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.5.2602-2607.2000"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.3.357"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90673-X"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1086\/515404"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2008.07.002"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.2387-2399.2005"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7558-7568.2005"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00017-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00017-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T20:08:02Z","timestamp":1646424482000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00017-11"}},"subtitle":[],"editor":[{"given":"J. N.","family":"Weiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,8]]},"references-count":49,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1128\/IAI.00017-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.00017-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,8]]},"assertion":[{"value":"2011-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-05-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-07-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}