{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T14:17:04Z","timestamp":1793888224403,"version":"4.8.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T00:00:00Z","timestamp":1710201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T00:00:00Z","timestamp":1710201600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2024,3,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Virus-like particles (VLPs) are protein-based nanoparticles frequently used as carriers in conjugate vaccine platforms. VLPs have been used to display foreign antigens for vaccination and to deliver immunotherapy against diseases. Hemolysin-coregulated proteins 1 (Hcp1) is a protein component of the\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      type 6 secretion system, which participates in intracellular invasion and dissemination. This protein has been reported as a protective antigen and is used in multiple vaccine candidates with various platforms against melioidosis, a severe infectious disease caused by the intracellular pathogen\n                      <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                      . In this study, we used P22 VLPs as a surface platform for decoration with Hcp1 using chemical conjugation. C57BL\/6 mice were intranasally immunized with three doses of either PBS, VLPs, or conjugated Hcp1-VLPs. Immunization with Hcp1-VLPs formulation induced Hcp1-specific IgG, IgG\n                      <jats:sub>1<\/jats:sub>\n                      , IgG\n                      <jats:sub>2c<\/jats:sub>\n                      , and IgA antibody responses. Furthermore, the serum from Hcp1-VLPs immunized mice enhanced the bacterial uptake and opsonophagocytosis by macrophages in the presence of complement. This study demonstrated an alternative strategy to develop a VLPs-based vaccine platform against\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      species.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00019-24","type":"journal-article","created":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T09:00:39Z","timestamp":1707901239000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Decoration of\n                    <i>Burkholderia<\/i>\n                    Hcp1 protein to virus-like particles as a vaccine delivery platform"],"prefix":"10.1128","volume":"92","author":[{"given":"Nittaya","family":"Khakhum","sequence":"first","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noe","family":"Baruch-Torres","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob L.","family":"Stockton","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7878-4499","authenticated-orcid":false,"given":"Itziar","family":"Chapartegui-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Badten","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Institute for Translational Sciences, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awadalkareem","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0156-6773","authenticated-orcid":true,"given":"Alejandro","family":"Huerta-Saquero","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Centro de Nanociencias y Nanotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ensenada, Baja California, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. 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