{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:42:22Z","timestamp":1787240542054,"version":"build-2736575974"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Gamma interferon (IFN-\u03b3) is a key cytokine in host defense against intracellular mycobacterial infection. It has been believed that both CD4 and CD8 T cells are the primary sources of IFN-\u03b3. However, the relative contributions of CD4 and CD8 T-cell subsets to IFN-\u03b3 production and the relationship between CD4 and CD8 T-cell activation have not been examined. By using a model of pulmonary mycobacterial infection and various immunodetection assays, we found that CD4 T cells mounted a much stronger IFN-\u03b3 response than CD8 T cells at various times after mycobacterial infection, and this pronounced IFN-\u03b3 production by CD4 T cells was attributed to both greater numbers of antigen-specific CD4 T cells and a greater IFN-\u03b3 secretion capacity of these cells. By using major histocompatibility complex class II-deficient or CD4-deficient mice, we found that the lack of CD4 T cells did not negatively affect primary or secondary CD8 T-cell IFN-\u03b3 responses. The CD8 T cells activated in the absence of CD4 T cells were capable of immune protection against secondary mycobacterial challenge. Our results suggest that, whereas both CD4 and CD8 T cells are capable of IFN-\u03b3 production, the former represent a much greater cellular source of IFN-\u03b3. Moreover, during mycobacterial infection, CD8 T-cell IFN-\u03b3 responses and activation are independent of CD4 T-cell activation.<\/jats:p>","DOI":"10.1128\/iai.00024-07","type":"journal-article","created":{"date-parts":[[2007,2,16]],"date-time":"2007-02-16T20:58:30Z","timestamp":1171659510000},"page":"2244-2252","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Gamma Interferon Responses of CD4 and CD8 T-Cell Subsets Are Quantitatively Different and Independent of Each Other during Pulmonary\n                    <i>Mycobacterium bovis<\/i>\n                    BCG Infection"],"prefix":"10.1128","volume":"75","author":[{"given":"Patricia","family":"Ngai","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"McCormick","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cherrie","family":"Small","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xizhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Zganiacz","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine and Division of Infectious Diseases, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"5407","DOI":"10.4049\/jimmunol.162.9.5407","volume":"162","year":"1999","unstructured":"Caruso, A. M., N. Serbina, E. Klein, K. Triebold, B. R. Bloom, and J. L. Flynn. 1999. Mice deficient in CD4 T cells have only transiently diminished levels of IFN-\u03b3, yet succumb to tuberculosis. J. Immunol.162:5407-5416.","journal-title":"J. Immunol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2243"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.4.1317-1320.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1685-1692.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/315466"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(01)02005-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.22.3.3776"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011347"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250211"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.1.127-132.1997"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.1107"},{"key":"e_1_3_2_13_2","first-page":"1448","volume":"216","year":"1993","unstructured":"Locksley, R. M., S. L. Reiner, F. Hatam, D. R. Littman, and N. Killeen. 1993. Helper T cells without CD4: control of leishmaniasis in CD4-deficient mice. Science216:1448-1451.","journal-title":"Science"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.3.271"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2004.03.004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-8749(02)00510-5"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4320-4328.2001"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.12.6991"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.2053"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2000.00936.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.830-834.1998"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4628-4637.2002"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031961"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"6101","DOI":"10.4049\/jimmunol.160.12.6101","volume":"160","year":"1998","unstructured":"Wakeham, J., J. Wang, J. Magram, K. Croitoru, R. Harkness, Dunn, A. P. Zganiacz, and Z. Xing. 1998. Lack of both types 1 and 2 cytokines, tissue inflammatory responses, and immune protection during pulmonary infection by Mycobacterium bovis bacillus Calmette-Gu\u00e9rin in IL-12-deficient mice. J. Immunol.160:6101-6111.","journal-title":"J. Immunol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6224"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.7.4590"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5537-5542.1998"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1189\/jlb.68.6.897","volume":"68","year":"2000","unstructured":"Xing, Z., A. Zganiacz, and M. Santosuosso. 2000. Role of IL-12 in macrophage activation during intracellular infection: IL-12 and mycobacteria synergistically release TNF-\u03b1 and nitric oxide from macrophages via IFN-\u03b3 induction. J. Leukoc. Biol.68:897-902.","journal-title":"J. Leukoc. Biol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.375"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI18991"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00024-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00024-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T19:44:14Z","timestamp":1707594254000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00024-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1128\/IAI.00024-07"],"URL":"https:\/\/doi.org\/10.1128\/iai.00024-07","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5]]},"assertion":[{"value":"2007-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}