{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T09:44:59Z","timestamp":1775900699420,"version":"3.50.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K08AI09532"],"award-info":[{"award-number":["K08AI09532"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001680","name":"Charles H. Hood Foundation","doi-asserted-by":"publisher","award":["Child Health Services Research Award"],"award-info":[{"award-number":["Child Health Services Research Award"]}],"id":[{"id":"10.13039\/100001680","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Loss-of-function mutations in the signal transducer and activator of transcription 3 gene (\n            <jats:italic>stat3<\/jats:italic>\n            ) result in autosomal dominant hyper-IgE syndrome (AD-HIES), a condition in which patients have recurrent debilitating infections, including frequent pneumococcal and staphylococcal pneumonias.\n            <jats:italic>stat3<\/jats:italic>\n            mutations cause defective adaptive T\n            <jats:sub>H<\/jats:sub>\n            17 cellular responses, an immune mechanism believed to be critical for clearance of pneumococcal colonization and diminished antibody responses. Here we wished to evaluate the role of\n            <jats:italic>stat3<\/jats:italic>\n            in the clearance of pneumococcal carriage and immunity using mice with a\n            <jats:italic>stat3<\/jats:italic>\n            mutation recapitulating AD-HIES. We show here that naive AD-HIES mice have prolonged nasal carriage of pneumococcus compared to WT mice. Mutant and wild-type mice were then immunized with a pneumococcal whole-cell vaccine (WCV) that provides T\n            <jats:sub>H<\/jats:sub>\n            17-mediated protection against pneumococcal colonization and antibody-mediated protection against pneumonia and sepsis. WCV-immunized AD-HIES mice made significantly less pneumococcus-specific interleukin-17A (IL-17A) and antibody than WT mice. The WCV-elicited protection against colonization was abrogated in AD-HIES mice, but immunization with WCV still protected AD-HIES mice against aspiration pneumonia\/sepsis. Taken together, our results suggest that impaired clearance of nasopharyngeal carriage due to poor adaptive IL-17A responses may contribute to the increased rates of pneumococcal respiratory infection in AD-HIES patients.\n          <\/jats:p>","DOI":"10.1128\/iai.00024-18","type":"journal-article","created":{"date-parts":[[2018,2,19]],"date-time":"2018-02-19T14:29:18Z","timestamp":1519050558000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of the Role of\n            <i>stat3<\/i>\n            in Antibody and T\n            <sub>H<\/sub>\n            17-Mediated Responses to Pneumococcal Immunization and Infection by Use of a Mouse Model of Autosomal Dominant Hyper-IgE Syndrome"],"prefix":"10.1128","volume":"86","author":[{"given":"Kristin","family":"Moffitt","sequence":"first","affiliation":[{"name":"Boston Children's Hospital, Division of Infectious Diseases, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine","family":"Cheung","sequence":"additional","affiliation":[{"name":"Boston Children's Hospital, Division of Infectious Diseases, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Manis","sequence":"additional","affiliation":[{"name":"Boston Children's Hospital, Joint Program in Transfusion Medicine, Department of Laboratory Medicine, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Malley","sequence":"additional","affiliation":[{"name":"Boston Children's Hospital, Division of Infectious Diseases, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200102083440602"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD004977.pub2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2015.1087625"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-280880-7.50014-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu274"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/319218"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0b013e31825f95b9"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa073687"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06096"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080321"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080218"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06764"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(88)90873-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2008.08.002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091706"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130323"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20082767"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2009.100"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.11.002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000159"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.09.031"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36731"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2187-2195.2006"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.01.007"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.43"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002989"},{"key":"e_1_3_2_28_2","volume-title":"Abstr 54th Intersci Conf Antimicrob Agents Chemother","author":"Skoberne M","year":"2014","unstructured":"Skoberne M, Morris MMA, Hopson K, Chan J, O'Toole M, Velez D, Siddall N, Hart M, Cooney M, Flechtner JB, Hetherington S. 2014. 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