{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:29:17Z","timestamp":1790418557966,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The Lyme disease spirochete,\n                    <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n                    , controls protein expression patterns during its tick-mammal infection cycle. Earlier studies demonstrated that\n                    <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n                    synthesizes 4,5-dihydroxy-2,3-pentanedione (autoinducer-2 [AI-2]) and responds to AI-2 by measurably changing production of several infection-associated proteins.\n                    <jats:italic>luxS<\/jats:italic>\n                    mutants, which are unable to produce AI-2, exhibit altered production of several proteins.\n                    <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n                    cannot utilize the other product of LuxS, homocysteine, indicating that phenotypes of\n                    <jats:italic>luxS<\/jats:italic>\n                    mutants are not due to the absence of that molecule. Although a previous study found that a\n                    <jats:italic>luxS<\/jats:italic>\n                    mutant was capable of infecting mice, a critical caveat to those results is that bacterial loads were not quantified. To more precisely determine whether LuxS serves a role in mammalian infection, mice were simultaneously inoculated with congenic wild-type and\n                    <jats:italic>luxS<\/jats:italic>\n                    strains, and bacterial numbers were assessed using quantitative PCR. The wild-type bacteria substantially outcompeted the mutants, suggesting that LuxS performs a significant function during mammalian infection. These data also provide further evidence that nonquantitative infection studies do not necessarily provide conclusive results and that regulatory factors may not make all-or-none, black-or-white contributions to infectivity.\n                  <\/jats:p>","DOI":"10.1128\/iai.00032-15","type":"journal-article","created":{"date-parts":[[2015,1,21]],"date-time":"2015-01-21T01:01:14Z","timestamp":1421802074000},"page":"1347-1353","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Apparent Role for Borrelia burgdorferi LuxS during Mammalian Infection"],"prefix":"10.1128","volume":"83","author":[{"given":"William K.","family":"Arnold","sequence":"first","affiliation":[{"name":"University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina R.","family":"Savage","sequence":"additional","affiliation":[{"name":"University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyssa D.","family":"Antonicello","sequence":"additional","affiliation":[{"name":"University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Stevenson","sequence":"additional","affiliation":[{"name":"University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-006-0690-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134040"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2714"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4099-4105.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(03)00184-9"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-4-36"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.9.3079-3087.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.12.011"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/004424-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5375.375"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1639"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/415545a"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02532.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00422.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.07.020"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03781.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02669.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1146"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/003350-0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.3122-3125.2002"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2892-2896.2003"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4864-4867.2004"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc12c01s4"},{"key":"e_1_3_2_26_2","first-page":"223","volume-title":"Lyme disease: molecular and immunologic approaches","author":"Barthold SW","year":"1992","unstructured":"Barthold SW, de Souza M, Fikrig E, Persing DH. 1992. Lyme borreliosis in the laboratory mouse, p 223\u2013242. In Schutzer SE (ed), Lyme disease: molecular and immunologic approaches. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00202-2"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.987-992.1999"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5953-5957.2001"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01496-4"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00521-12"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00219-13"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02035-05"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.05042.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000169"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834315100"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06621.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.12.002"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12171"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01199.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(03)00028-6"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.50.1.727"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.4839-4849.2000"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.42.352"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.4.883"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.53.397"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4145-4148.2001"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025351727785"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01956-12"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39401"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02400-9"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00285-004-0296-0"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00032-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00032-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T04:57:42Z","timestamp":1646456262000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00032-15"}},"subtitle":[],"editor":[{"given":"A. J.","family":"B\u00e4umler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":51,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1128\/IAI.00032-15"],"URL":"https:\/\/doi.org\/10.1128\/iai.00032-15","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"2015-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-01-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}