{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:53:41Z","timestamp":1792205621370,"version":"4.4.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previous studies have demonstrated that the\n            <jats:italic>ebp<\/jats:italic>\n            operon and the\n            <jats:italic>ace<\/jats:italic>\n            gene of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterococcus faecalis<\/jats:named-content>\n            , encoding\n            <jats:underline>e<\/jats:underline>\n            ndocarditis- and\n            <jats:underline>b<\/jats:underline>\n            iofilm-associated\n            <jats:underline>p<\/jats:underline>\n            ili and an\n            <jats:underline>a<\/jats:underline>\n            dhesin to\n            <jats:underline>c<\/jats:underline>\n            ollagen of\n            <jats:underline>\n              <jats:italic>E<\/jats:italic>\n            <\/jats:underline>\n            <jats:italic>. faecalis<\/jats:italic>\n            , respectively, are both important in experimental urinary tract infections (UTI) and endocarditis. We have also shown that growth of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. faecalis<\/jats:named-content>\n            in brain heart infusion (BHI) serum enhances Ebp pilus and Ace production and increases adherence to several host extracellular matrix proteins. Here, we report that deletion of\n            <jats:italic>ebpABC<\/jats:italic>\n            almost eliminated serum-elicited adherence to fibrinogen (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001), resulted in moderate reduction in adherence to collagen (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05), and had no effect on fibronectin adherence relative to that of wild-type OG1RF. An OG1RF\u0394\n            <jats:italic>ace<\/jats:italic>\n            \u0394\n            <jats:italic>ebpABC<\/jats:italic>\n            double mutant showed further reduced collagen adherence versus that of the OG1RF\u0394\n            <jats:italic>ace<\/jats:italic>\n            or OG1RF\u0394\n            <jats:italic>ebpABC<\/jats:italic>\n            mutants (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). These results were corroborated by complementation and\/or studies with native pilus-enriched surface extracts and a collagen-secreting 3T6 fibroblast cell line, as well as antibody inhibition. In the UTI model, both the OG1RF\u0394\n            <jats:italic>ace<\/jats:italic>\n            and OG1RF\u0394\n            <jats:italic>ace<\/jats:italic>\n            \u0394\n            <jats:italic>ebpABC<\/jats:italic>\n            mutants were found to be significantly attenuated compared to the wild type; however, no significant differences were observed between individual\n            <jats:italic>ace<\/jats:italic>\n            or\n            <jats:italic>ebp<\/jats:italic>\n            mutants and the OG1RF\u0394\n            <jats:italic>ace<\/jats:italic>\n            \u0394\n            <jats:italic>ebpABC<\/jats:italic>\n            mutant. In summary, these data implicate the Ebp pili as having some role in collagen adherence, albeit less than that of Ace, and a very major role in fibrinogen adherence, which may explain in part the importance of these pili in experimental endocarditis. The OG1RF\u0394\n            <jats:italic>ace<\/jats:italic>\n            \u0394\n            <jats:italic>ebpABC<\/jats:italic>\n            mutant was attenuated in the UTI model, although not significantly more so than the \u0394\n            <jats:italic>ace<\/jats:italic>\n            or \u0394\n            <jats:italic>ebpABC<\/jats:italic>\n            mutants, suggesting involvement of other\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. faecalis<\/jats:named-content>\n            factors in urinary tract colonization or infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00038-11","type":"journal-article","created":{"date-parts":[[2011,4,20]],"date-time":"2011-04-20T03:08:41Z","timestamp":1303268921000},"page":"2901-2910","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":100,"title":["Relative Contributions of Ebp Pili and the Collagen Adhesin Ace to Host Extracellular Matrix Protein Adherence and Experimental Urinary Tract Infection by Enterococcus faecalis OG1RF"],"prefix":"10.1128","volume":"79","author":[{"given":"Sreedhar R.","family":"Nallapareddy","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030"},{"name":"Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavindra V.","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030"},{"name":"Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jouko","family":"Sillanp\u00e4\u00e4","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030"},{"name":"Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030"},{"name":"Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030"},{"name":"Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara E.","family":"Murray","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030"},{"name":"Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030"},{"name":"Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp0804651"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.6.5.637"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.9.1899"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-7-r110"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.24.8161-8163.2004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiq160"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00725-10"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555817923"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00711-10"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00219-09"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00275-09"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.06.004"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH06-11-0640"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000026"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.2003.00059.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0402.980211"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2009.10.019"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.2461-2468.2005"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00663-07"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2010.03.002"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01837-08"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000422"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2006.08.003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01218-08"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01153-06"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.10.010"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05704.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3658-3663.2004"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507808102"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(01)00009-5"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.3.1.46"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.9.2059-2063.1990"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1381.1999.99241.x"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00772-08"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00039-11"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00476-06"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1086\/588143"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5218-5224.2000"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI29021"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03417.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07190.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1086\/591182"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00405-08"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01241.x"},{"key":"e_1_3_3_47_2","first-page":"223","article-title":"MSCRAMM\u2014targeted vaccines and immunotherapy for staphylococcal infection","volume":"7","author":"Rivas J. M.","year":"2004","unstructured":"RivasJ. M.SpezialeP.PattiJ. M.HookM.. 2004. MSCRAMM\u2014targeted vaccines and immunotherapy for staphylococcal infection. Curr. Opin. Drug Discov. Devel. 7:223\u2013227.","journal-title":"Curr. Opin. Drug Discov. Devel."},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04887.x"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.027821-0"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/017319-0"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.1.4.11966"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27074-0"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1998.tb01180.x"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1086\/600124"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1086\/517524"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000716"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.37"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.102"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.04.014"},{"key":"e_1_3_3_60_2","first-page":"2.4.1","volume-title":"Current protocols in molecular biology","author":"Wilson K","year":"1994","unstructured":"WilsonK. 1994. Preparation of genomic DNA from bacteria, p. 2.4.1\u20132.4.2. In AusubelF. M.BrentR.KingstonR. E.DavidD. M.ScidmanJ. G.SmithJ. A.StruhlK. (ed.),Current protocols in molecular biology. Green Publishing Associates, Brooklyn, NY."},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1606-1612.2005"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1149-1154.2004"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00038-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00038-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:48:59Z","timestamp":1646488139000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00038-11"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2011,7]]},"references-count":61,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1128\/IAI.00038-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.00038-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7]]},"assertion":[{"value":"2011-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-06-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}