{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:04:31Z","timestamp":1791266671544,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>is an obligate intracellular Gram-negative bacterium that causes the zoonotic disease Q fever. Although Q fever is mainly transmitted by aerosol infection, study of the immune responses in the lung following pulmonary<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection is lacking. Neutrophils are considered the first immune cell to migrate into the lung and play an important role in host defense against aerosol infection with microbial pathogens. However, the role of neutrophils in the host defense against<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection remains unclear. To determine the role of neutrophils in protective immunity against<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection, the RB6-8C5 antibody was used to deplete neutrophils in mice before intranasal infection with<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>. The results indicated that neutrophil-depleted mice developed more severe disease than their wild-type counterparts, suggesting that neutrophils play an important role in host defense against<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>pulmonary infection. We also found that neither CXC chemokine receptor 2 (CXCR2) nor interleukin-17 (IL-17) receptor (IL-17R) deficiency changed the severity of disease following intranasal<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>challenge, suggesting that keratinocyte-derived chemokine and IL-17 may not play essential roles in the response to<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection. However, significantly higher<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>genome copy numbers were detected in the lungs of IL-1R<jats:sup>\u2212\/\u2212<\/jats:sup>mice at 14 days postinfection. This indicates that IL-1 may be important for the clearance of<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>from the lungs following intranasal infection. Our results also suggest that neutrophils are involved in protecting vaccinated mice from<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>challenge-induced disease. This is the first study to demonstrate an important role for neutrophils in protective immunity against<jats:named-content content-type=\"genus-species\">C. burnetii<\/jats:named-content>infection.<\/jats:p>","DOI":"10.1128\/iai.00042-15","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T15:24:51Z","timestamp":1432740291000},"page":"3104-3113","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Neutrophils Play an Important Role in Protective Immunity against Coxiella burnetii Infection"],"prefix":"10.1128","volume":"83","author":[{"given":"Alexandra","family":"Elliott","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Schoenlaub","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Freches","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri\u2014Columbia, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.25.3.285-293.1961"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00293.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0047TR"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00973-13"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0031-6997(24)01456-X","article-title":"Neutrophil migration mechanisms, with an emphasis on the pulmonary vasculature","volume":"52","author":"Wagner JG","year":"2000","unstructured":"Wagner JG, Roth RA. 2000. Neutrophil migration mechanisms, with an emphasis on the pulmonary vasculature. Pharm Rev 52:349\u2013374.","journal-title":"Pharm Rev"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01169-09"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198902093200606"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01487042"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.538"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.5.3355"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.3.851-858.1986"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00297-13"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2194"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc06c01s15"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0407247"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00101-10"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1401s83"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.3.1091-1102.1982"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200909-1427SO"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12096"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00406-10"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.6.3695"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2075-2080.2004"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.04.005"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu430"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300100"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003565"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00111"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.9.4432"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.25.3.4424"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100001"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00042-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00042-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T03:39:55Z","timestamp":1748403595000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00042-15"}},"subtitle":[],"editor":[{"given":"C. R.","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1128\/IAI.00042-15"],"URL":"https:\/\/doi.org\/10.1128\/iai.00042-15","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8]]},"assertion":[{"value":"2015-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}