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The Eurasian badger (<jats:italic>Meles meles<\/jats:italic>) represents a wildlife source of recurrent<jats:italic>M. bovis<\/jats:italic>infections of cattle in the United Kingdom, and its vaccination against TB with<jats:italic>M. bovis<\/jats:italic>bacillus Calmette-Gue\u0301rin (BCG) is an attractive disease control option. Delivery of BCG in oral bait holds the best prospect for vaccinating badgers over a wide geographical area. Using a guinea pig pulmonary challenge model, we evaluated the protective efficacy of candidate badger oral vaccines, based on broth-grown or ball-milled BCG, delivered either as aqueous suspensions or formulated in two lipids with differing fatty acid profiles (one being animal derived and the other being vegetable derived). Protection was determined in terms of increasing body weight after aerosol challenge with virulent<jats:italic>M. bovis<\/jats:italic>, reduced dissemination of<jats:italic>M. bovis<\/jats:italic>to the spleen, and, in the case of one oral formulation, restricted growth of<jats:italic>M. bovis<\/jats:italic>in the lungs. Only oral BCG formulated in lipid gave significant protection. These data point to the potential of the BCG-lipid formulation for further development as a tool for controlling tuberculosis in badgers.<\/jats:p>","DOI":"10.1128\/iai.00052-08","type":"journal-article","created":{"date-parts":[[2008,6,3]],"date-time":"2008-06-03T00:57:02Z","timestamp":1212454622000},"page":"3771-3776","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Oral Vaccination of Guinea Pigs with a<i>Mycobacterium bovis<\/i>Bacillus Calmette-Gue\u0301rin Vaccine in a Lipid Matrix Protects against Aerosol Infection with Virulent<i>M. bovis<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Simon","family":"Clark","sequence":"first","affiliation":[{"name":"Health Protection Agency, Porton Down, Salisbury SP4 0JG"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin L.","family":"Cross","sequence":"additional","affiliation":[{"name":"Centre for Innovation, University of Otago, P.O. 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