{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T07:15:57Z","timestamp":1778224557241,"version":"3.51.4"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Wuhan City Health Bureau Project","award":["WX20Q22"],"award-info":[{"award-number":["WX20Q22"]}]},{"name":"Wuhan City Science and Techology Bureau Project","award":["2022020801020540"],"award-info":[{"award-number":["2022020801020540"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              To research the role of the NLRP3 inflammasome in\n              <jats:italic>Schistosoma japonicum<\/jats:italic>\n              -induced granuloma formation and liver fibrosis. In\n              <jats:italic>in vivo<\/jats:italic>\n              tests, BALB\/c mice were used. shNLRP3 plasmid based on adeno-associated virus serotype 8 (AAV8-shNLRP3) was injected to block NLRP3 inflammasome via tail vein. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were detected to assess liver injury. H&amp;E staining was used for routine histopathological assessment; Masson\u2019s trichrome staining was used to detect fibrous tissues and collagen fibers. Hepatic expression of NLRP3, procaspase-1, bioactive caspase-1, collagen-1, tissue inhibitor of metalloproteinases-1 (TIMP-1), and \u03b1-smooth muscle actin (\u03b1-SMA) were detected by western blot. Serum levels of IL-1\u03b2 were detected by enzyme-linked immunosorbent assay (ELISA). The inflammatory cell infiltration and hepatic expression of IL-1\u03b2 around the granuloma were detected by immunohistochemistry staining. Treatment of\n              <jats:italic>S. japonicum<\/jats:italic>\n              infected mice with AAV8-shNLRP3 significantly reduced the hepatic levels of bioactive caspase-1 and IL-1\u03b2, as well as circulating IL-1\u03b2 concentrations, while reducing the amounts of myeloperoxidase (MPO) and F4\/80 positive cells around the granuloma. Moreover, collagen deposition, TIMP-1, and \u03b1-SMA, which are markers of hepatic stellate cell (HSC) activation, were reduced around the liver granuloma. These findings highlight a therapeutic potential of AAV8-shNLRP3 in schistosomiasis cirrhosis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00055-24","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T12:49:29Z","timestamp":1724071769000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["NLRP3 inflammasome inhibition decreases\n            <i>Schistosomiasis japonica<\/i>\n            -induced granulomatous inflammation and fibrosis in BALB\/c mice"],"prefix":"10.1128","volume":"92","author":[{"given":"Yaqi","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 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