{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:09:07Z","timestamp":1776424147619,"version":"3.51.2"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI080615"],"award-info":[{"award-number":["R01AI080615"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI116620"],"award-info":[{"award-number":["R01AI116620"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["P01AI138949"],"award-info":[{"award-number":["P01AI138949"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["1ZIATR000355-04"],"award-info":[{"award-number":["1ZIATR000355-04"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2022,5,19]]},"abstract":"<jats:p>\n            The\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            BB0323 protein undergoes a complex yet poorly defined proteolytic maturation event that generates N-terminal and C-terminal proteins with essential functions in cell growth and infection. Here, we report that a borrelial protease,\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            high temperature requirement A protease (BbHtrA), cleaves BB0323 between asparagine (N) and leucine (L) at positions 236 and 237, while the replacement of these residues with alanine in the mutant protein prevents its cleavage, despite preserving its normal secondary structure.\n          <\/jats:p>","DOI":"10.1128\/iai.00059-22","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:01:47Z","timestamp":1649854907000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Controlled Proteolysis of an Essential Virulence Determinant Dictates Infectivity of Lyme Disease Pathogens"],"prefix":"10.1128","volume":"90","author":[{"given":"Meghna","family":"Thakur","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandhya","family":"Bista","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelby D.","family":"Foor","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shraboni","family":"Dutta","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuli","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Ronzetti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"},{"name":"National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vipin S.","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chrysoula","family":"Kitsou","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara B.","family":"Linden","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda S.","family":"Altieri","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bolormaa","family":"Baljinnyam","sequence":"additional","affiliation":[{"name":"National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3248-4831","authenticated-orcid":true,"given":"Daniel C.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"},{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland, USA"},{"name":"Virginia-Maryland College of Veterinary Medicine, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Simeonov","sequence":"additional","affiliation":[{"name":"National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7504-4628","authenticated-orcid":true,"given":"Utpal","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"},{"name":"Virginia-Maryland College of Veterinary Medicine, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2015.02.010"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2714"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21681"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)01146-5"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01698.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033280"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12882"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032667699"},{"key":"e_1_3_3_11_2","first-page":"467","volume-title":"Borrelia, molecular biology, host interaction and pathogenesis.","author":"Marconi RT","year":"2010","unstructured":"Marconi RT, Earnhart CG. 2010. Lyme disease vaccines, p 467\u2013486. In Samuels DS, Radolf JD (ed), Borrelia, molecular biology, host interaction and pathogenesis. Caister Academic Press, Norfolk, United Kingdom."},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz872"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.5973-5979.2004"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu460"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12202"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/605846"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06211.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5269"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.18.4872-4878.1998"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.17.4764-4772.2000"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00607-10"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319010751"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13594"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2019.06.009"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2019.129499"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a018259"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04016.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03279-7"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00360-16"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13221"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01136-18"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12213"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14243"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12377"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01977-14"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1610805113"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.7.2074-2084.2004"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3065"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(02)00658-5"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/416455a"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2014.03.011"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.17.9642"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07591.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01288-08"},{"key":"e_1_3_3_45_2","doi-asserted-by":"crossref","unstructured":"Motaleb MA Corum L Bono JL Elias AF Rosa P Samuels DS Charon NW. 2000. Borrelia burgdorferi periplasmic flagella have both skeletal and motility functions. Proc Natl Acad Sci USA 97:10899\u201310904.","DOI":"10.1073\/pnas.200221797"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06613.x"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00214"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)97551-4"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4245-4254.2000"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00501-10"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12487"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00059-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00059-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T13:03:26Z","timestamp":1652965406000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.00059-22"}},"subtitle":[],"editor":[{"given":"Guy H.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,5,19]]},"references-count":50,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5,19]]}},"alternative-id":["10.1128\/iai.00059-22"],"URL":"https:\/\/doi.org\/10.1128\/iai.00059-22","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,19]]},"assertion":[{"value":"2022-02-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00059-22"}}