{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T02:24:28Z","timestamp":1783131868494,"version":"3.54.6"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,4,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Brucella abortus<\/jats:italic>\n              infects the placenta of its natural bovine host, which results in abortion and transmission of infection to other cattle and to humans. While the metabolism of\n              <jats:italic>B. abortus<\/jats:italic>\n              during chronic infection of the mononuclear phagocyte system has been studied, the nutrients fueling growth of\n              <jats:italic>B. abortus<\/jats:italic>\n              in the placenta are unknown. We found that in mice, glucose is an important carbon source for\n              <jats:italic>B. abortus<\/jats:italic>\n              in the placenta. A\n              <jats:italic>gluP<\/jats:italic>\n              mutant lacking a major facilitator superfamily protein required for glucose uptake had diminished growth in the placenta of pregnant mice and caused reduced inflammatory pathology and fetal demise. The\n              <jats:italic>gluP<\/jats:italic>\n              mutant was able to replicate intracellularly in a trophoblast cellular model and to cause trophoblast cell death in infected placentas. Attenuated growth of the\n              <jats:italic>gluP<\/jats:italic>\n              mutant was maintained in mice conditionally deficient for peroxisome proliferator-activated receptor \u03b3 in macrophages, suggesting that M2-like macrophages were not the major site for glucose-dependent growth of\n              <jats:italic>B. abortus<\/jats:italic>\n              in the placenta. Our results show that the infected placenta contains multiple distinct nutrient niches and that glucose utilization within the interstitial space of the placenta is an important process contributing to bacterial growth and fetal demise during placental\n              <jats:italic>B. abortus<\/jats:italic>\n              infection.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00060-25","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T13:00:56Z","timestamp":1741784456000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Glucose uptake is essential for\n            <i>Brucella abortus<\/i>\n            growth in the extracellular space of the murine placenta"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7720-5627","authenticated-orcid":true,"given":"Leticia","family":"L\u00e1zaro-Ant\u00f3n","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaynara Parente de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California","place":["Davis, USA"]},{"name":"Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1rias, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais","place":["Belo Horizonte, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Pirillo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2122-4156","authenticated-orcid":true,"given":"Mariana X.","family":"Byndloss","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center","place":["Nashville, USA"]},{"name":"Howard Hughes Medical Institute, Vanderbilt University Medical Center","place":["Nashville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir E.","family":"Diaz-Ochoa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Briana M.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4830-0470","authenticated-orcid":true,"given":"Renato de Lima","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1rias, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais","place":["Belo Horizonte, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-6657","authenticated-orcid":true,"given":"Ren\u00e9e M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.04.007"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0965539515000066"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.11.006"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2009.04.010"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2008.10.004"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-016-0092-0"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/740479"},{"key":"e_1_3_4_9_2","first-page":"361","article-title":"Colonization of mouse placentas by Brucella abortus inoculated during pregnancy","volume":"61","author":"Bosseray N","year":"1980","unstructured":"Bosseray N. 1980. 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