{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:17:55Z","timestamp":1761059875329,"version":"3.40.5"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Botulinum neurotoxins (BoNTs) possess unique specificity for nerve terminals. They bind to the presynaptic membrane and then translocate intracellularly, where the light-chain endopeptidase cleaves the SNARE complex proteins, subverting the synaptic exocytosis responsible for acetylcholine release to the synaptic cleft. This inhibits acetylcholine binding to its receptor, causing paralysis. Binding, an obligate event for cell intoxication, is believed to occur through the heavy-chain C-terminal (H\n            <jats:sub>C<\/jats:sub>\n            ) domain. It is followed by toxin translocation and entry into the cell cytoplasm, which is thought to be mediated by the heavy-chain N-terminal (H\n            <jats:sub>N<\/jats:sub>\n            ) domain. Submolecular mapping analysis by using synthetic peptides spanning BoNT serotype A (BoNT\/A) and mouse brain synaptosomes (SNPs) and protective antibodies against toxin from mice and cervical dystonia patients undergoing BoNT\/A treatment revealed that not only regions of the H\n            <jats:sub>C<\/jats:sub>\n            domain but also regions of the H\n            <jats:sub>N<\/jats:sub>\n            domain are involved in the toxin binding process. Based on these findings, we expressed a peptide corresponding to the BoNT\/A region comprising H\n            <jats:sub>N<\/jats:sub>\n            domain residues 729 to 845 (H\n            <jats:sub>N<\/jats:sub>\n            729\u2013845). H\n            <jats:sub>N<\/jats:sub>\n            729\u2013845 bound directly to mouse brain SNPs and substantially inhibited BoNT\/A binding to SNPs. The binding involved gangliosides GT1b and GD1a and a few membrane lipids. The peptide bound to human or mouse neuroblastoma cells within 1 min. Peptide H\n            <jats:sub>N<\/jats:sub>\n            729\u2013845 protected mice completely against a lethal BoNT\/A dose (1.05 times the 100% lethal dose). This protective activity was obtained at a dose comparable to that of the peptide from positions 967 to 1296 in the H\n            <jats:sub>C<\/jats:sub>\n            domain. These findings strongly indicate that H\n            <jats:sub>N<\/jats:sub>\n            729\u2013845 and, by extension, the H\n            <jats:sub>N<\/jats:sub>\n            domain are fully programmed and equipped to bind to neuronal cells and in the free state can even inhibit the binding of the toxin.\n          <\/jats:p>","DOI":"10.1128\/iai.00063-15","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T02:33:40Z","timestamp":1422326020000},"page":"1465-1476","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["The C-Terminal Heavy-Chain Domain of Botulinum Neurotoxin A Is Not the Only Site That Binds Neurons, as the N-Terminal Heavy-Chain Domain Also Plays a Very Active Role in Toxin-Cell Binding and Interactions"],"prefix":"10.1128","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6775-9546","authenticated-orcid":false,"given":"B. Vijayalakshmi","family":"Ayyar","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Roger","family":"Aoki","sequence":"additional","affiliation":[{"name":"Neurotoxin Research Program, Department of Biological Sciences, Allergan, Inc., Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Zouhair","family":"Atassi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA"},{"name":"Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.8.1059"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(99)80047-6"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.2.717"},{"key":"e_1_3_3_5_2","first-page":"219","article-title":"Structure, activity, and immune (T and B cell) recognition of botulinum neurotoxins","volume":"19","author":"Atassi MZ","year":"1999","unstructured":"Atassi MZ, Oshima M. 1999. Structure, activity, and immune (T and B cell) recognition of botulinum neurotoxins. 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