{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:15:01Z","timestamp":1767960901148,"version":"3.49.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Salivary proline-rich proteins (PRPs) attach commensal\n            <jats:italic>Actinomyces<\/jats:italic>\n            and\n            <jats:italic>Streptococcus<\/jats:italic>\n            species to teeth. Here, gel filtration, mass spectrometry and Edman degradation were applied to show the release of a pentapeptide, RGRPQ, from PRP-1 upon proteolysis by\n            <jats:italic>Streptococcus gordonii<\/jats:italic>\n            . Moreover, synthetic RGRPQ and derivatives were used to investigate associated innate properties and responsible motifs. The RGRPQ peptide increased 2.5-fold the growth rate of\n            <jats:italic>S. gordonii<\/jats:italic>\n            via a Q-dependent sequence motif and selectively stimulated oral colonization of this organism in a rat model in vivo. In contrast, the growth of\n            <jats:italic>Streptococcus mutans<\/jats:italic>\n            , implicated in caries, was not affected. While the entire RGRPQ sequence was required to block sucrose-induced pH-decrease by\n            <jats:italic>S. gordonii<\/jats:italic>\n            and\n            <jats:italic>S. mutans<\/jats:italic>\n            , the N-terminal Arg residue mediated the pH increase (i.e., ammonia production) by\n            <jats:italic>S. gordonii<\/jats:italic>\n            alone (which exhibits Arg catabolism to ammonia). Strains of commensal viridans streptococci exhibited PRP degradation and Arg catabolism, whereas cariogenic species did not. The RGRPQ peptide mediated via a differential Q-dependent sequence motif, adhesion inhibition, and desorption of PRP-1-binding strains of\n            <jats:italic>A. naeslundii<\/jats:italic>\n            genospecies 2 (5 of 10 strains) but not of\n            <jats:italic>S. gordonii<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 5). The inhibitable\n            <jats:italic>A. naeslundii<\/jats:italic>\n            strains alone displayed the same binding profile as\n            <jats:italic>S. gordonii<\/jats:italic>\n            to hybrid peptides terminating in RGRPQ or GQSPQ, derived from the middle or C-terminal segments of PRP-1. The present findings indicate the presence of a host-bacterium interaction in which a host peptide released by bacterial proteolysis affects key properties in biofilm formation.\n          <\/jats:p>","DOI":"10.1128\/iai.00068-06","type":"journal-article","created":{"date-parts":[[2006,10,20]],"date-time":"2006-10-20T22:57:47Z","timestamp":1161385067000},"page":"6293-6299","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Host-Derived Pentapeptide Affecting Adhesion, Proliferation, and Local pH in Biofilm Communities Composed of\n            <i>Streptococcus<\/i>\n            and\n            <i>Actinomyces<\/i>\n            Species"],"prefix":"10.1128","volume":"74","author":[{"given":"Mirva","family":"Drobni","sequence":"first","affiliation":[{"name":"Department of Odontology\/Cariology, Umea\u030a University, SE-901 87 Umea\u030a, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Odontology\/Cariology, Umea\u030a University, SE-901 87 Umea\u030a, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carina","family":"Kru\u0308ger","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vuokko","family":"Loimaranta","sequence":"additional","affiliation":[{"name":"Department of Odontology\/Cariology, Umea\u030a University, SE-901 87 Umea\u030a, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mogens","family":"Kilian","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Aarhus University, DK-8000 Aarhus C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lennart","family":"Hammarstro\u0308m","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Jo\u0308rnvall","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Bergman","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicklas","family":"Stro\u0308mberg","sequence":"additional","affiliation":[{"name":"Department of Odontology\/Cariology, Umea\u030a University, SE-901 87 Umea\u030a, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.19.4413"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790041401"},{"key":"e_1_3_2_4_2","first-page":"141","volume":"17","year":"1988","unstructured":"Azen, E. A., and N. Maeda. 1988. Molecular genetics of human salivary proteins and their polymorphisms. Adv. Hum. Genet.17:141-199.","journal-title":"Adv. Hum. Genet."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.1001-1009.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/337385a0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.2.643-652.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2000.917301.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345880670010301"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680090101"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09393.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.21.1.41-47.1978"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/69.5.1052s"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000016628"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1983.tb07456.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm907"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680050101"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.2.439-445.1988"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.9.2948-2954.1991"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001773200"},{"key":"e_1_3_2_21_2","first-page":"327","volume":"13","year":"1988","unstructured":"Hallberg, K., K.-J. Hammarstr\u00f6m, E. Falsen, G. Dahlen, R. Gibbons, D. Hay, and N. Str\u00f6mberg. 1988. Actinomyces naeslundii genospecies 1 and 2 express different binding specificities to N-acetyl-\u03b2-d-galactosamine, whereas Actinomyces odontolyticus expresses a different binding specificity in colonizing the human mouth. Oral Microbiol. Immunol.13:327-336.","journal-title":"Oral Microbiol. Immunol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.521416.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345940730110701"},{"key":"e_1_3_2_24_2","first-page":"125","volume":"7","year":"1994","unstructured":"Hsu, S. D., J. O. Cisar, A. L. Sandberg, and M. Kilian. 1994. Adhesive properties of viridans streptococcal species. Microb. Ecol. Health Dis.7:125-137.","journal-title":"Microb. Ecol. Health Dis."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12055"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac010486h"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/5213"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-39-4-471"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0702-702"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00395933"},{"key":"e_1_3_2_31_2","first-page":"251","volume":"4","year":"1993","unstructured":"Lamkin, M. S., and F. G. Oppenheim. 1993. Structural features of salivary function. Crit. Rev. Oral Biol. Med.4:251-259.","journal-title":"Crit. Rev. Oral Biol. Med."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01313-4"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5425-5429.2000"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7224-7233.2001"},{"key":"e_1_3_2_35_2","first-page":"557","volume":"3","year":"2000","unstructured":"Liu, B., S. A. Rayment, C. Gyurko, F. G. Oppenheim, G. D. Offner, and R. F. Troxler. 2000. The recombinant N-terminal region of human salivary mucin MG2 (MUC7) contains a binding domain for oral Streptococci and exhibits candidacidal activity. Biochem. J. 345 Pt.3:557-564.","journal-title":"Biochem. J. 345 Pt."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.15.6868"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2004.01010.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68522-9"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.44.3.702-707.1984"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006928200"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411940050030201"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800111101"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1994.tb00061.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1996.tb00484.x"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.11.4.649-655.1975"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.354-362.2005"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90045-9"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00068-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00068-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:16:47Z","timestamp":1646461007000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00068-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":46,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1128\/IAI.00068-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.00068-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]},"assertion":[{"value":"2006-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-08-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}