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The rapid transfer of<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>from infected dendritic cells to effector natural killer (NK) cells may contribute to the parasite's sequestration and shielding from immune recognition shortly after infection. However, subversion of NK cell functions, such as cytotoxicity or production of proinflammatory cytokines, such as gamma interferon (IFN-\u03b3), upon parasite infection might also be beneficial to the parasite. In the present study, we investigated the effects of<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>infection on NK cells.<jats:italic>In vitro<\/jats:italic>, infected NK cells were found to be poor at killing target cells and had reduced levels of IFN-\u03b3 production. This could be attributed in part to the inability of infected cells to form conjugates with their target cells. However, even upon NK1.1 cross-linking of NK cells, the infected NK cells also exhibited poor degranulation and IFN-\u03b3 production. Similarly, NK cells infected<jats:italic>in vivo<\/jats:italic>were also poor at killing target cells and producing IFN-\u03b3. Increased levels of transforming growth factor \u03b2 production, as well as increased levels of expression of SHP-1 in the cytosol of infected NK cells upon infection, were observed in infected NK cells. However, the phosphorylation of STAT4 was not altered in infected NK cells, suggesting that transcriptional regulation mediates the reduced IFN-\u03b3 production, which was confirmed by quantitative PCR. These data suggest that infection of NK cells by<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>impairs NK cell recognition of target cells and cytokine release, two mechanisms that independently could enhance<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>survival.<\/jats:p>","DOI":"10.1128\/iai.00069-17","type":"journal-article","created":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:30:29Z","timestamp":1501547429000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Downmodulation of Effector Functions in NK Cells upon Toxoplasma gondii Infection"],"prefix":"10.1128","volume":"85","author":[{"given":"M. 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