{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:53:13Z","timestamp":1790110393264,"version":"4.0.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Candida glabrata<\/jats:named-content>\n            is an emerging opportunistic pathogen that is known to develop resistance to azole drugs due to increased drug efflux. The mechanism consists of\n            <jats:italic>CgPDR1<\/jats:italic>\n            -mediated upregulation of ATP-binding cassette transporters. A range of gain-of-function (GOF) mutations in\n            <jats:italic>CgPDR1<\/jats:italic>\n            have been found to lead not only to azole resistance but also to enhanced virulence. This implicates\n            <jats:italic>CgPDR1<\/jats:italic>\n            in the regulation of the interaction of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. glabrata<\/jats:named-content>\n            with the host. To identify specific\n            <jats:italic>CgPDR1<\/jats:italic>\n            -regulated steps of the host-pathogen interaction, we investigated in this work the interaction of selected\n            <jats:italic>CgPDR1<\/jats:italic>\n            GOF mutants with murine bone marrow-derived macrophages and human acute monocytic leukemia cell line (THP-1)-derived macrophages, as well as different epithelial cell lines. GOF mutations in\n            <jats:italic>CgPDR1<\/jats:italic>\n            did not influence survival and replication within macrophages following phagocytosis but led to decreased adherence to and uptake by macrophages. This may allow evasion from the host's innate cellular immune response. The interaction with epithelial cells revealed an opposite trend, suggesting that GOF mutations in\n            <jats:italic>CgPDR1<\/jats:italic>\n            may favor epithelial colonization of the host by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. glabrata<\/jats:named-content>\n            through increased adherence to epithelial cell layers. These data reveal that GOF mutations in\n            <jats:italic>CgPDR1<\/jats:italic>\n            modulate the interaction with host cells in ways that may contribute to increased virulence.\n          <\/jats:p>","DOI":"10.1128\/iai.00074-13","type":"journal-article","created":{"date-parts":[[2013,3,5]],"date-time":"2013-03-05T10:22:14Z","timestamp":1362478934000},"page":"1709-1720","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Gain-of-Function Mutations in\n            <i>PDR1<\/i>\n            , a Regulator of Antifungal Drug Resistance in Candida glabrata, Control Adherence to Host Cells"],"prefix":"10.1128","volume":"81","author":[{"given":"Lu\u00eds","family":"Vale-Silva","sequence":"first","affiliation":[{"name":"Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Ischer","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salom\u00e9","family":"Leibundgut-Landmann","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Swiss Federal Institute of Technology, Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Sanglard","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408410903241444"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01283-12"},{"key":"e_1_3_2_4_2","article-title":"Is the incidence of candidemia caused by Candida glabrata increasing in Brazil? 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