{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:03:06Z","timestamp":1789758186672,"version":"4.0.1"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The infection of nonphagocytic host cells by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n                    and more particularly by small-colony variants (SCVs) may contribute to the persistence of this pathogen in the lungs of cystic fibrosis (CF) patients. The development of chronic infections is also thought to be facilitated by the proinflammatory status of CF airways induced by an activation of NF-\u03baB. The aim of this study was to compare the infection of non-CF and CF-like airway epithelial cells by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    strains (normal and SCVs) and to determine the impact of the interaction between cystic fibrosis transmembrane conductance regulator (CFTR) and NF-\u03baB on the infection level of these cells by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    . We developed an\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    infection model using polarized airway epithelial cells grown at the air-liquid interface and expressing short hairpin RNAs directed against CFTR to mimic the CF condition. A pair of genetically related CF coisolates with the normal and SCV phenotypes was characterized and used. Infection of both cell lines (non-CF and CF-like) was more productive with the SCV strain than with its normal counterpart. However, both normal and SCV strains infected more CF-like than non-CF cells. Accordingly, inhibition of CFTR function by CFTR\n                    <jats:italic>inh<\/jats:italic>\n                    -172 increased the\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    infection level. Experimental activation of NF-\u03baB also increased the level of infection of polarized pulmonary epithelial cells by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    , an event that could be associated with that observed when CFTR function is inhibited or impaired. This study supports the hypothesis that the proinflammatory status of CF tissues facilitates the infection of pulmonary epithelial cells by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/iai.00078-11","type":"journal-article","created":{"date-parts":[[2011,6,28]],"date-time":"2011-06-28T02:56:41Z","timestamp":1309229801000},"page":"3541-3551","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Infection of Polarized Airway Epithelial Cells by Normal and Small-Colony Variant Strains of Staphylococcus aureus Is Increased in Cells with Abnormal Cystic Fibrosis Transmembrane Conductance Regulator Function and Is Influenced by NF-\u03baB"],"prefix":"10.1128","volume":"79","author":[{"given":"Gabriel","family":"Mitchell","sequence":"first","affiliation":[{"name":"Centre d'\u00c9tude et de Valorisation de la Diversit\u00e9 Microbienne (CEVDM), D\u00e9partement de Biologie, Facult\u00e9 des Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1K 2R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Grondin","sequence":"additional","affiliation":[{"name":"Centre d'\u00c9tude et de Valorisation de la Diversit\u00e9 Microbienne (CEVDM), D\u00e9partement de Biologie, Facult\u00e9 des Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1K 2R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginette","family":"Bilodeau","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Pulmonaire, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 M.","family":"Cantin","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Pulmonaire, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Malouin","sequence":"additional","affiliation":[{"name":"Centre d'\u00c9tude et de Valorisation de la Diversit\u00e9 Microbienne (CEVDM), D\u00e9partement de Biologie, Facult\u00e9 des Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1K 2R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100703"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/520289"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2008.0110"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.11.030"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.4.1033"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2003.12.007"},{"key":"e_1_3_2_8_2","unstructured":"Canadian Cystic Fibrosis Foundation. 2007. 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