{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T23:04:21Z","timestamp":1791759861318,"version":"4.3.4"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Virulent\n            <jats:named-content content-type=\"genus-species\">Streptococcus suis<\/jats:named-content>\n            serotype 2 strains are invasive extracellular bacteria causing septicemia and meningitis in piglets and humans. One objective of this study was to elucidate the function of complement in innate immune defense against\n            <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n            . Experimental infection of wild-type (WT) and C3\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice demonstrated for the first time that the complement system protects naive mice against invasive mucosal\n            <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n            infection.\n            <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n            WT but not an unencapsulated mutant caused mortality associated with meningitis and other pathologies in C3\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice. The capsule contributed also substantially to colonization of the upper respiratory tract. Experimental infection of C3\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice with a suilysin mutant indicated that suilysin expression facilitated an early disease onset and the pathogenesis of meningitis. Flow cytometric analysis revealed C3 antigen deposition on the surface of ca. 40% of\n            <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n            WT bacteria after opsonization with naive WT mouse serum, although to a significantly lower intensity than on the unencapsulated mutant.\n            <jats:italic>Ex vivo<\/jats:italic>\n            multiplication in murine WT and C3\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            blood depended on capsule but not suilysin expression. Interestingly,\n            <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n            invasion of inner organs was also detectable in C5aR\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice, suggesting that chemotaxis and activation of immune cells via the anaphylatoxin receptor C5aR is, in addition to opsonization, a further important function of the complement system in defense against mucosal\n            <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n            infection. In conclusion, we unequivocally demonstrate here the importance of complement against mucosal\n            <jats:named-content content-type=\"genus-species\">S. suis<\/jats:named-content>\n            serotype 2 infection and that the capsule of this pathogen is also involved in escape from complement-independent immunity.\n          <\/jats:p>","DOI":"10.1128\/iai.00080-14","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T04:23:04Z","timestamp":1396326184000},"page":"2460-2471","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Role of Capsule and Suilysin in Mucosal Infection of Complement-Deficient Mice with Streptococcus suis"],"prefix":"10.1128","volume":"82","author":[{"given":"Maren","family":"Seitz","sequence":"first","affiliation":[{"name":"Institute for Microbiology, University of Veterinary Medicine Hanover, Hanover, 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