{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:21:22Z","timestamp":1790518882772,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The polysaccharide capsule is known to be the major factor required for the virulence of\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            . We have cloned and characterized a gene, designated\n            <jats:italic>CPS1<\/jats:italic>\n            , that encodes a protein containing a glycosyltransferase moiety and shares similarity with the type 3 polysaccharide synthase encoded by the\n            <jats:italic>cap3B<\/jats:italic>\n            gene of\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            . Cps1p also shares similarity with hyaluronan synthase of higher eukaryotes. Deletion of the\n            <jats:italic>CPS1<\/jats:italic>\n            gene from a serotype D strain of\n            <jats:italic>C. neoformans<\/jats:italic>\n            resulted in a slight reduction of the capsule size as observed by using an India ink preparation. The growth at 37\u00b0C was impaired, and the ability to associate with human brain endothelial cells in vitro was also significantly reduced by the deletion of\n            <jats:italic>CPS1<\/jats:italic>\n            . Using site-specific mutagenesis, we showed that the conserved glycosyltransferase domains are critical for the ability of the strain to grow at elevated temperatures. A hyaluronan enzyme-linked immunosorbent assay method demonstrated that\n            <jats:italic>CPS1<\/jats:italic>\n            is important for the synthesis of hyaluronan or its related polysaccharides in\n            <jats:italic>C. neoformans<\/jats:italic>\n            . Comparisons between the wild-type and the\n            <jats:italic>cps1\u0394<\/jats:italic>\n            strains, using three different transmission electron microscopic methods, indicated that the\n            <jats:italic>CPS1<\/jats:italic>\n            gene product is involved in the composition or maintenance of an electron-dense layer between the outer cell wall and the capsule. These and the virulence studies in a mouse model suggested that the\n            <jats:italic>CPS1<\/jats:italic>\n            gene is important in the pathobiology of\n            <jats:italic>C. neoformans<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00089-06","type":"journal-article","created":{"date-parts":[[2006,6,21]],"date-time":"2006-06-21T23:10:29Z","timestamp":1150931429000},"page":"3930-3938","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["<i>CPS1<\/i>\n            , a Homolog of the\n            <i>Streptococcus pneumoniae<\/i>\n            Type 3 Polysaccharide Synthase Gene, Is Important for the Pathobiology of\n            <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1128","volume":"74","author":[{"given":"Y. 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