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Epidemiological studies indicate that the frequency of diarrhea outbreaks caused by ATEC is increasing. Hence, it is of major importance to further characterize putative factors contributing to the pathogenicity of these strains and to gain additional insight into the plasticity and evolutionary aspects of this emerging pathotype. Here, we analyzed the two clinical ATEC isolates B6 (O26:K60) and 9812 (O128:H2) and compared the genetic organizations, flanking regions, and chromosomal insertion loci of their LEE with those of the LEE of other A\/E pathogens. Our analysis shows that the core LEE is largely conserved\u2014particularly among genes coding for the type 3 secretion system\u2014whereas genes encoding effector proteins display a higher variability. Chromosomal insertion loci appear to be restricted to<jats:italic>selC<\/jats:italic>,<jats:italic>pheU<\/jats:italic>, and<jats:italic>pheV<\/jats:italic>. In contrast, striking differences were found between the 5\u2032- and 3\u2032-associated flanking regions reflecting the different histories of the various strains and also possibly indicating different lines in evolution.<\/jats:p>","DOI":"10.1128\/iai.00090-09","type":"journal-article","created":{"date-parts":[[2009,6,9]],"date-time":"2009-06-09T01:28:56Z","timestamp":1244510936000},"page":"3501-3513","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Comparative Analysis of the Locus of Enterocyte Effacement and Its Flanking Regions"],"prefix":"10.1128","volume":"77","author":[{"given":"Daniel","family":"Mu\u0308ller","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Infektiologie, Zentrum fu\u0308r Molekularbiologie der Entzu\u0308ndung (ZMBE)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inga","family":"Benz","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Infektiologie, Zentrum fu\u0308r Molekularbiologie der Entzu\u0308ndung (ZMBE)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Liebchen","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Infektiologie, Zentrum fu\u0308r Molekularbiologie der Entzu\u0308ndung (ZMBE)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inka","family":"Gallitz","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Infektiologie, Zentrum fu\u0308r Molekularbiologie der Entzu\u0308ndung (ZMBE)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helge","family":"Karch","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Hygiene, Westfa\u0308lische Wilhelms-Universita\u0308t Mu\u0308nster\/Universita\u0308tsklinikum Mu\u0308nster, D-48149 Mu\u0308nster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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