{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:37:11Z","timestamp":1785908231894,"version":"3.56.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100011684","name":"Crohn's and Colitis Foundation","doi-asserted-by":"publisher","award":["854385"],"award-info":[{"award-number":["854385"]}],"id":[{"id":"10.13039\/100011684","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["855256"],"award-info":[{"award-number":["855256"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"NIH National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK134053"],"award-info":[{"award-number":["DK134053"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001424","name":"American Liver Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001424","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,8,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Malaria, caused by\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      spp., is a global health concern linked to anemia and increased mortality. Compensatory erythropoiesis seen during acute anemia results in an increased circulating reticulocyte count (i.e., immature RBC), a key factor in understanding the relationship between pre-existing anemia and\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      burden. Reticulocytes in mice are marked by transferrin receptor (CD71\n                      <jats:sup>+<\/jats:sup>\n                      ) and glycophorin A-associated protein (Ter119\n                      <jats:sup>+<\/jats:sup>\n                      ). To model acute anemia with increased reticulocytes, C57BL\/6J mice were either bled (i.e., phlebotomized) or administered phenylhydrazine before being infected with\n                      <jats:italic toggle=\"yes\">Plasmodium yoelii<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      ), a mouse-specific strain with a preference for reticulocytes. In\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      -infected anemic mice, we observed heightened parasitemia and significant body weight loss compared with non-anemic\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      -infected mice. Additionally, serum inflammatory cytokines, erythropoietin, and liver injury markers, along with hemozoin deposition, significantly increased in anemic\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      -infected mice. Blood transfusion from healthy normal donors to\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      -infected anemic recipient mice ameliorated anemia by reducing overall reticulocyte count and increasing mature RBC count. Blood transfusion rescued body weight loss, decreased parasitemia, and reduced serum erythropoietin levels. Finally, to confirm the role of reticulocytes in\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      infection, reticulocytes were depleted using anti-CD71 monoclonal antibody in\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      -infected mice. We observed improvement in hematologic parameters and stark reduction in parasitemia in both pre-existing anemic and non-anemic\n                      <jats:italic toggle=\"yes\">P. yoelii<\/jats:italic>\n                      -infected mice. Collectively, our results suggest that pre-existing anemia may increase the risk of\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      infection due to the greater reticulocyte population. Anti-CD71 treatment in\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      infection may offer a novel therapeutic strategy to combat\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      infection and malaria.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00093-25","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:59:42Z","timestamp":1751363982000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Targeting anemia-induced CD71\n                    <sup>+<\/sup>\n                    reticulocytes protects mice from\n                    <i>Plasmodium<\/i>\n                    infection"],"prefix":"10.1128","volume":"93","author":[{"given":"Sareh","family":"Zeydabadinejad","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Hypertension and Precision Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Sung Anthony","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Zheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mrunmayee Rajendra","family":"Kandalgaonkar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Hypertension and Precision Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prince Boakye","family":"Ababio","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Gohara","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01600wh70","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology, University of Toledo Medical Center","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matam","family":"Vijay-Kumar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Hypertension and Precision Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beng","family":"San Yeoh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Hypertension and Precision Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0626-398X","authenticated-orcid":true,"given":"Piu","family":"Saha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbdzh19","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Hypertension and Precision Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences","place":["Toledo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-08-596015"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1022828"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000133"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPCRK3MOAOJ6AT"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2018.06.004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1003614"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2009.1.87"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2509-9"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232112762"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021015055"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2017.10.031"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046101"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a025494"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-106"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5954394"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00025"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom13081262"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2015.05.011"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1471-4922(02)02353-x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01555.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD001475"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(20)30288-X"},{"key":"e_1_3_3_24_2","first-page":"1","article-title":"Exchange transfusion in severe falciparum malaria","volume":"82","author":"Gulprasutdilog S","year":"1999","unstructured":"Gulprasutdilog S, Chongkolwatana V, Buranakitjaroen P, Jaroonvesama N. 1999. Exchange transfusion in severe falciparum malaria. J Med Assoc Thai 82:1\u20138.","journal-title":"J Med Assoc Thai"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2005-0314"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21249713"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.680136"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqae009"},{"key":"e_1_3_3_29_2","first-page":"235","article-title":"Cytokine dysregulation associated with malarial anemia in Plasmodium yoelii infected mice","volume":"5","author":"Xu L","year":"2013","unstructured":"Xu L, Zheng X, Berzins K, Chaudhuri A. 2013. Cytokine dysregulation associated with malarial anemia in Plasmodium yoelii infected mice. Am J Transl Res 5:235\u2013245.","journal-title":"Am J Transl Res"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5446"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2016.11.011"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.9.3761-3765.1994"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760220184"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91499-3"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.607692"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1097\/CJI.0b013e318222ffc8"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abb0130"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan1078"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00939-3"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm7348"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004670"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01613"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.44.1.151-156.1984"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit553"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2006.09.008"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12576"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V65.4.886.886"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00093-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00093-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T13:00:57Z","timestamp":1755003657000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.00093-25"}},"subtitle":[],"editor":[{"given":"Jeroen P. J.","family":"Saeij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,8,12]]},"references-count":46,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,12]]}},"alternative-id":["10.1128\/iai.00093-25"],"URL":"https:\/\/doi.org\/10.1128\/iai.00093-25","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.01.13.632761","asserted-by":"object"}]},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,12]]},"assertion":[{"value":"2025-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00093-25"}}