{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T02:13:59Z","timestamp":1778811239648,"version":"3.51.4"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP22K09950"],"award-info":[{"award-number":["JP22K09950"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP20K10286, JP24K02661"],"award-info":[{"award-number":["JP20K10286, JP24K02661"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,3,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic toggle=\"yes\">Streptococcus mutans<\/jats:italic>\n              , a cariogenic bacterium in humans, is associated with systemic disorders. Its cariogenic factors include glucosyltransferases (GTFs) and the glycosyltransferase rhamnose\u2013glucose polysaccharide I (RgpI), which is involved in cell wall synthesis. However, the potential roles of these enzymes in systemic disorders remain unclear. We constructed a luciferase-tagged\n              <jats:italic toggle=\"yes\">S. mutans<\/jats:italic>\n              UA159 mutant strain that lacked\n              <jats:italic toggle=\"yes\">rgpI<\/jats:italic>\n              to explore the involvement of this enzyme in the systemic pathogenicity of\n              <jats:italic toggle=\"yes\">S. mutans<\/jats:italic>\n              . We also employed the luciferase-tagged\n              <jats:italic toggle=\"yes\">S. mutans<\/jats:italic>\n              UA159 variant, which exhibited reduced GTF production and therefore had a low glucan synthesis ability. We intravenously inoculated these luciferase-tagged mutants and parent strains into 12-week-old male BALB\/c mice to evaluate their distribution to organs. Strong luminescence was noted in the spleen and kidneys, indicating that\n              <jats:italic toggle=\"yes\">S. mutans<\/jats:italic>\n              was disseminated to these organs. Several organs collected from mice inoculated with the luciferase-tagged parent strain emitted a signal, and inflammatory cytokine production was detected in the blood. The luminescence intensity was lower in the kidneys of mice challenged with the mutant strain, which has a low glucan synthesis ability. Conversely, challenge with the\n              <jats:italic toggle=\"yes\">rgpI<\/jats:italic>\n              deletion mutant strain resulted in the lowest number of luminescent organs, with a lower intensity and attenuated inflammation. Furthermore, all the mice inoculated with the\n              <jats:italic toggle=\"yes\">rgpI<\/jats:italic>\n              deletion mutant strain survived, whereas not all the mice inoculated with the parent strain survived. Collectively, these results suggest that RgpI is involved in the systemic pathogenicity of\n              <jats:italic toggle=\"yes\">S. mutans<\/jats:italic>\n              UA159.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00097-24","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T14:02:21Z","timestamp":1740060141000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Cell wall glycosyltransferase of\n            <i>Streptococcus mutans<\/i>\n            impacts its dissemination to murine organs"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6323-9535","authenticated-orcid":true,"given":"Tomomi","family":"Hashizume-Takizawa","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo","place":["Matsudo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiki","family":"Ando","sequence":"additional","affiliation":[{"name":"Department of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University","place":["Tokyo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayaka","family":"Urakawa","sequence":"additional","affiliation":[{"name":"Department of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University","place":["Tokyo, Japan"]},{"name":"Department of Bacteriology I, National Institute of Infectious Diseases","place":["Tokyo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University","place":["Tokyo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hedenobu","family":"Senpuku","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo","place":["Matsudo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1977.03630210045014"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.44.2.331-384.1980"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000324598"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.9.4.892-897.2002"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1273-1283.2006"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3261-3270.2005"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104260"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000070447"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.198-202.2004"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.11336\/7940"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.64"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14330"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.9.3261-3266.1991"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00497-17"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2006.00317.x"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02247-18"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1491"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02164-14"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02343-06"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47051-0"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/83.2.456S"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11451.x"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12953"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.11.008"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-020-03217-4"},{"key":"e_1_3_4_27_2","unstructured":"Urakawa A. 2020. 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