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The organism possesses multiple genes encoding autotransporter proteins, which represent important virulence factors and targets for developing countermeasures in pathogenic Gram-negative bacteria. In the present study, we investigated one of these autotransporters, BatA, and demonstrate that it displays lipolytic activity, aids in intracellular survival, is expressed\n            <jats:italic>in vivo<\/jats:italic>\n            , elicits production of antibodies during infection, and contributes to pathogenicity in a mouse aerosol challenge model. A mutation in the\n            <jats:italic>batA<\/jats:italic>\n            gene of wild-type strain ATCC 23344 was found to be particularly attenuating, as BALB\/c mice infected with the equivalent of 80 median lethal doses cleared the organism. This finding prompted us to test the hypothesis that vaccination with the\n            <jats:italic>batA<\/jats:italic>\n            mutant strain elicits protective immunity against subsequent infection with wild-type bacteria. We discovered that not only does vaccination provide high levels of protection against lethal aerosol challenge with\n            <jats:named-content content-type=\"genus-species\">B. mallei<\/jats:named-content>\n            ATCC 23344, it also protects against infection with multiple isolates of the closely related organism and causative agent of melioidosis,\n            <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n            . Passive-transfer experiments also revealed that the protective immunity afforded by vaccination with the\n            <jats:italic>batA<\/jats:italic>\n            mutant strain is predominantly mediated by IgG antibodies binding to antigens expressed exclusively\n            <jats:italic>in vivo<\/jats:italic>\n            . Collectively, our data demonstrate that BatA is a target for developing medical countermeasures and that vaccination with a mutant lacking expression of the protein provides a platform to gain insights regarding mechanisms of protective immunity against\n            <jats:named-content content-type=\"genus-species\">B. mallei<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            , including antigen discovery.\n          <\/jats:p>","DOI":"10.1128\/iai.00102-17","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T00:50:17Z","timestamp":1494895817000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Antibodies against\n            <i>In Vivo<\/i>\n            -Expressed Antigens Are Sufficient To Protect against Lethal Aerosol Infection with Burkholderia mallei and Burkholderia pseudomallei"],"prefix":"10.1128","volume":"85","author":[{"given":"Shawn M.","family":"Zimmerman","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy S.","family":"Dyke","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomislav P.","family":"Jelesijevic","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"},{"name":"Department of Veterinary Biosciences and Diagnostic Imaging, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Michel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences and Diagnostic Imaging, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric R.","family":"Lafontaine","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"},{"name":"Department of Veterinary Biosciences and Diagnostic Imaging, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.8"},{"key":"e_1_3_3_3_2","first-page":"147","volume-title":"Textbooks of biological warfare series","author":"Vietri NJ","year":"2007","unstructured":"Vietri NJ Deshazer D . 2007. 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