{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:47:24Z","timestamp":1777596444446,"version":"3.51.4"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Apicomplexan parasites include those of the genera\n            <jats:named-content content-type=\"genus-species\">Plasmodium<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            and those of the relatively understudied zoonotic genus\n            <jats:named-content content-type=\"genus-species\">Babesia<\/jats:named-content>\n            . In humans, babesiosis, particularly transfusion-transmitted babesiosis, has been emerging as a major threat to public health. Like malaria, the disease pathology is a consequence of the parasitemia which develops through cyclical replication of\n            <jats:named-content content-type=\"genus-species\">Babesia<\/jats:named-content>\n            parasites in host erythrocytes. However, there are no exoerythrocytic stages in\n            <jats:named-content content-type=\"genus-species\">Babesia<\/jats:named-content>\n            , so targeting of the blood stage and associated proteins to directly prevent parasite invasion is the most desirable option for effective disease control. Especially promising among such molecules are the rhoptry neck proteins (RONs), whose homologs have been identified in many apicomplexan parasites. RONs are involved in the formation of the moving junction, along with AMA1, but no RON has been identified and characterized in any\n            <jats:named-content content-type=\"genus-species\">Babesia<\/jats:named-content>\n            spp. Here we identify the RON2 proteins of\n            <jats:named-content content-type=\"genus-species\">Babesia divergens<\/jats:named-content>\n            (BdRON2) and\n            <jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>\n            (BmRON2) and show that they are localized apically and that anti-BdRON2 antibodies are significant inhibitors of parasite invasion\n            <jats:italic>in vitro<\/jats:italic>\n            . Neither protein is immunodominant, as both proteins react only marginally with sera from infected animals. Further characterization of the direct role of both BdRON2 and BmRON2 in parasite invasion is required, but knowledge of the level of conformity of RON2 proteins within the apicomplexan phylum, particularly that of the AMA1-RON2 complex at the moving junction, along with the availability of an animal model for\n            <jats:named-content content-type=\"genus-species\">B. microti<\/jats:named-content>\n            studies, provides a key to target this complex with a goal of preventing the erythrocytic invasion of these parasites and to further our understanding of the role of these conserved ligands in invasion.\n          <\/jats:p>","DOI":"10.1128\/iai.00107-16","type":"journal-article","created":{"date-parts":[[2016,3,8]],"date-time":"2016-03-08T03:38:27Z","timestamp":1457408307000},"page":"1574-1584","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Identification and Characterization of the Rhoptry Neck Protein 2 in Babesia divergens and B. microti"],"prefix":"10.1128","volume":"84","author":[{"given":"Rosalynn L.","family":"Ord","sequence":"first","affiliation":[{"name":"Department of Blood-Borne Parasites, New York Blood Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilis","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Blood-Borne Parasites, New York Blood Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeny R.","family":"Cursino-Santos","sequence":"additional","affiliation":[{"name":"Department of Blood-Borne Parasites, New York Blood Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyunryung","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Blood-Borne Parasites, New York Blood Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manpreet","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Blood-Borne Parasites, New York Blood Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Gray","sequence":"additional","affiliation":[{"name":"University College Dublin, School of Biology and Environmental Science, Dublin, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl A.","family":"Lobo","sequence":"additional","affiliation":[{"name":"Department of Blood-Borne Parasites, New York Blood Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(80)90232-1"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.4.622-636.2003"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(81)80020-2"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2015.02.008"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.3109\/01913120903251643"},{"key":"e_1_3_1_7_2","first-page":"505","article-title":"2011. 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