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In the context of\n                      <jats:italic toggle=\"yes\">Echinococcus granulosus<\/jats:italic>\n                      infection, parasite antigens can modulate host immune responses; however, their specific impact on DC metabolism remains poorly defined. We measured mitochondrial membrane potential (\u0394\u03a8M), intracellular reactive oxygen species (ROS), and nitric oxide (NO) production in FMS-like tyrosine kinase 3 ligand (FLT3-L)-derived DC (FL-DCs) stimulated with hydatid fluid (HF) or purified laminar layer (pLL) from\n                      <jats:italic toggle=\"yes\">E. granulosus<\/jats:italic>\n                      . Gene expression profiling of key metabolic enzymes involved in glycolysis and oxidative phosphorylation (OXPHOS) was performed using RT-qPCR. Functional metabolic flux was analyzed using the Seahorse glycolysis stress test, while key metabolites from both pathways were quantified by HPLC. Our results showed that HF-stimulated FL-DCs exhibited marked mitochondrial dysfunction, evidenced by reduced \u0394\u03a8M and diminished mitochondrial network complexity, in contrast to the preserved mitochondrial morphology in pLL-stimulated cells. Gene expression analysis revealed that both stimuli enhanced glycolytic enzyme transcripts; however, only pLL induced OXPHOS-related genes, suggesting divergent bioenergetic adaptations. HF-stimulated FL-DCs produced elevated levels of ROS and NO, indicative of oxidative stress and a glycolysis-favored metabolic state, whereas pLL maintained mitochondrial respiration without excessive ROS production. Metabolic flux assays corroborated these findings; HF-stimulated cells displayed an increased extracellular acidification rate and a decreased oxygen consumption rate, indicating a glycolytic shift. In contrast, pLL-stimulated FL-DCs preserved oxidative metabolism and aerobic glycolysis. Our findings demonstrate that HF and pLL from\n                      <jats:italic toggle=\"yes\">E. granulosus<\/jats:italic>\n                      differentially modulate metabolic programs in FL-DCs and highlight potential targets for modulating DC function in echinococcosis pathogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00107-26","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T14:00:33Z","timestamp":1789135233000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Mitochondrial dysfunction and glycolytic shift define the metabolic profile of hydatid fluid-stimulated dendritic cells"],"prefix":"10.1128","volume":"94","author":[{"given":"Maia","family":"Chop","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/055eqsb67","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto IQUIBIM, Facultad de 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