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We recently described the feasibility of a CRISPR interference (CRISPRi) system to inducibly repress gene expression in\n                    <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n                    . However, the initial system suffered from some drawbacks, primarily leaky expression of the anhydrotetracycline (aTc)-inducible dCas9 ortholog and plasmid instability, which prevented population-wide studies (e.g., transcript analyses) of the effects of knockdown.\n                  <\/jats:p>","DOI":"10.1128\/iai.00108-21","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T10:56:55Z","timestamp":1618916215000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["CRISPR Interference To Inducibly Repress Gene Expression in Chlamydia trachomatis"],"prefix":"10.1128","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3721-6839","authenticated-orcid":true,"given":"Scot P.","family":"Ouellette","sequence":"first","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel A.","family":"Blay","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan D.","family":"Hatch","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Fisher-Marvin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"370","article-title":"CDC Grand Rounds: Chlamydia prevention: challenges and strategies for reducing disease burden and sequelae","volume":"60","author":"Satterwhite C","year":"2011","unstructured":"Satterwhite C, Gottlieb S, Romaguera R, Bolan G, Burstein G, Schuler C, Popovic T. 2011. 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