{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:24:25Z","timestamp":1773404665983,"version":"3.50.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Septicemia caused by<jats:italic>Neisseria meningitidis<\/jats:italic>is characterized by increasing levels of meningococcal lipopolysaccharide (Nm-LPS) and cytokine production in the blood. We have used an in vitro human whole-blood model of meningococcal septicemia to investigate the potential of CyP, a selective Toll-like receptor 4 (TLR4)-MD-2 antagonist derived from the cyanobacterium<jats:italic>Oscillatoria planktothrix<\/jats:italic>FP1, for reducing LPS-mediated cytokine production. CyP (\u22651 \u03bcg\/ml) inhibited the secretion of the proinflammatory cytokines tumor necrosis factor alpha, interleukin-1\u03b2 (IL-1\u03b2), and IL-6 (by &gt;90%) and chemokines IL-8 and monocyte chemoattractant protein 1 (by \u223c50%) induced by the treatment of blood with pure Nm-LPS, by isolated outer membranes, and after infection with live meningococci of different serogroups. In vitro studies with human dendritic cells and TLR4-transfected Jurkat cells demonstrated that CyP competitively inhibited Nm-LPS interactions with TLR4 and subsequent NF-\u03baB activation. These data demonstrate that CyP is a potent antagonist of meningococcal LPS and could be considered a new adjunctive therapy for treating septicemia.<\/jats:p>","DOI":"10.1128\/iai.00110-08","type":"journal-article","created":{"date-parts":[[2008,4,29]],"date-time":"2008-04-29T00:53:38Z","timestamp":1209430418000},"page":"3156-3163","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["A Cyanobacterial Lipopolysaccharide Antagonist Inhibits Cytokine Production Induced by<i>Neisseria meningitidis<\/i>in a Human Whole-Blood Model of Septicemia"],"prefix":"10.1128","volume":"76","author":[{"given":"Kim","family":"Jemmett","sequence":"first","affiliation":[{"name":"Neisseria Research Group, Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalisa","family":"Macagno","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine, Via Vincenzo Vela 6, CH-6500 Bellinzona, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Molteni","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Molecular Sciences, University of Insubria, Via Dunant 3, 21100 Varese, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John E.","family":"Heckels","sequence":"additional","affiliation":[{"name":"Neisseria Research Group, Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Molecular Sciences, University of Insubria, Via Dunant 3, 21100 Varese, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myron","family":"Christodoulides","sequence":"additional","affiliation":[{"name":"Neisseria Research Group, Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"261","volume":"12","year":"2006","unstructured":"Andr\u00e4, J., T. 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