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Acute systemic infection strongly impacts MZ populations and the location of T and B lymphocytes. This phenomenon has been linked to reduced chemokine secretion by stromal cells.<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>spp. are the causative agent of brucellosis, a widespread zoonotic disease. Here, we used<jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>infection as a model to investigate the impact of chronic stealth infection on splenic MZ macrophage populations. During the late phase of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>infection, we observed a loss of both MZMs and MMMs, with a durable disappearance of MZMs, leading to a reduction of the ability of the spleen to take up soluble antigens, beads, and unrelated bacteria. This effect appears to be selective as every other lymphoid and myeloid population analyzed increased during infection, which was also observed following<jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">Brucella suis<\/jats:named-content>infection. Comparison of wild-type and deficient mice suggested that MZ macrophage population loss is dependent on interferon gamma (IFN-\u03b3) receptor but independent of T cells or tumor necrosis factor alpha receptor 1 (TNF-\u03b1R1) signaling pathways and is not correlated to an alteration of CCL19, CCL21, and CXCL13 chemokine mRNA expression. Our results suggest that MZ macrophage populations are particularly sensitive to persistent low-level IFN-\u03b3-mediated inflammation and that<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>infection could reduce the ability of the spleen to perform certain MZM- and MMM-dependent tasks, such as antigen delivery to lymphocytes and control of systemic infection.<\/jats:p>","DOI":"10.1128\/iai.00115-17","type":"journal-article","created":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T00:50:19Z","timestamp":1502758219000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen"],"prefix":"10.1128","volume":"85","author":[{"given":"Arnaud","family":"Machelart","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Universit\u00e9 de Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abir","family":"Khadrawi","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Universit\u00e9 de Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurore","family":"Demars","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Universit\u00e9 de Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Willemart","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Universit\u00e9 de Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"De Trez","sequence":"additional","affiliation":[{"name":"Research Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel and Vlaams Instituut voor Biotechnologie, Department of Structural Biology Research Center, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Universit\u00e9 de Namur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Muraille","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Universit\u00e9 Libre de Bruxelles, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.10.010"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00480"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307124101"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg019"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"939","DOI":"10.4049\/jimmunol.162.2.939","article-title":"Regulation and functional involvement of macrophage scavenger receptor MARCO in clearance of bacteria in vivo","volume":"162","author":"van der Laan LJ","year":"1999","unstructured":"van der Laan LJ Ea D\u00f6pp Haworth R Pikkarainen T Kangas M Elomaa O Dijkstra CD Gordon S Tryggvason K Kraal G . 1999. 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