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MAIT cells are activated by riboflavin metabolite derivatives presented by the nonpolymorphic major histocompatibility complex class I (MHC-I)-like molecule MR1. The precise mechanisms required to activate MAIT cells are an area of intense interest. Here we used two closely related intracellular pathogens with distinct inflammasome activation phenotypes to probe the role of innate cytokines in MAIT cell activation. Using an<jats:italic>in vitro<\/jats:italic>assay containing transgenic murine MAIT cells, we show that macrophages infected with<jats:named-content content-type=\"genus-species\">Francisella novicida<\/jats:named-content>, a strong inflammasome activator, released high levels of interleukin-18 (IL-18) and stimulated high levels of MAIT cell gamma interferon (IFN-\u03b3) through a partially MR1-independent pathway. In contrast, macrophages infected with<jats:named-content content-type=\"genus-species\">Francisella tularensis<\/jats:named-content>live vaccine strain (LVS), a weak inflammasome activator, generated little IL-18 and stimulated low MAIT cell IFN-\u03b3 through an MR1-dependent pathway. By manipulating the quantities of IL-18 in these cultures, we show that the IL-18 concentration is sufficient to influence the magnitude of MAIT cell IFN-\u03b3 production. Correspondingly, infected IL-18-deficient macrophages failed to induce substantial MAIT cell IFN-\u03b3. In contrast, we found that MAIT cell IFN-\u03b3 production in the lungs of IL-18-deficient mice was not significantly different from that in WT mice during<jats:named-content content-type=\"genus-species\">F. tularensis<\/jats:named-content>LVS pulmonary infection. Overall, we demonstrate that while IL-18 is essential for the MAIT cell IFN-\u03b3 response<jats:italic>in vitro<\/jats:italic>, it is not essential for MAIT cell IFN-\u03b3 production during<jats:italic>in vivo<\/jats:italic>LVS pulmonary infection, suggesting that additional signals can drive MAIT cell IFN-\u03b3 production<jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/iai.00117-18","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T09:54:12Z","timestamp":1520330052000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Interleukin-18 Is Critical for Mucosa-Associated Invariant T Cell Gamma Interferon Responses to Francisella Species<i>In Vitro<\/i>but Not<i>In Vivo<\/i>"],"prefix":"10.1128","volume":"86","author":[{"given":"Eric","family":"Jesteadt","sequence":"first","affiliation":[{"name":"Laboratory of Mycobacterial Diseases and Cellular Immunology, 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