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We have previously shown that the limitation of branched-chain amino acids (BCAAs) is a cue that induces the expression of a subset of<jats:italic>A. pleuropneumoniae<\/jats:italic>genes identified as specifically induced during infection of the natural host animal by using an in vivo expression technology screen. Leucine-responsive regulatory protein (Lrp) is a global regulator and has been shown in<jats:italic>Escherichia coli<\/jats:italic>to regulate many genes, including genes involved in BCAA biosynthesis. We hypothesized that<jats:italic>A. pleuropneumoniae<\/jats:italic>contains a regulator similar to Lrp and that this protein is involved in the regulation of a subset of genes important during infection and recently shown to have increased expression in the absence of BCAAs. We report the identification of an<jats:italic>A. pleuropneumoniae<\/jats:italic>serotype 1 gene encoding a protein with similarity to amino acid sequence and functional domains of other reported Lrp proteins. We further show that purified<jats:italic>A. pleuropneumoniae<\/jats:italic>His<jats:sub>6<\/jats:sub>-Lrp binds in vitro to the<jats:italic>A. pleuropneumoniae<\/jats:italic>promoter regions for<jats:italic>ilvI<\/jats:italic>, antisense<jats:italic>cps1AB<\/jats:italic>,<jats:italic>lrp<\/jats:italic>, and<jats:italic>nqr<\/jats:italic>. A genetically defined<jats:italic>A. pleuropneumoniae lrp<\/jats:italic>mutant was constructed using an allelic replacement and sucrose counterselection method. Analysis of expression from the<jats:italic>ilvI<\/jats:italic>and antisense<jats:italic>cps1AB<\/jats:italic>promoters in wild-type,<jats:italic>lrp<\/jats:italic>mutant, and complemented<jats:italic>lrp<\/jats:italic>mutant strains indicated that Lrp is required for induction of expression of<jats:italic>ilvI<\/jats:italic>under BCAA limitation.<\/jats:p>","DOI":"10.1128\/iai.00120-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T23:51:25Z","timestamp":1166658685000},"page":"91-103","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Identification of the<i>Actinobacillus pleuropneumoniae<\/i>Leucine-Responsive Regulatory Protein and Its Involvement in the Regulation of In Vivo-Induced Genes"],"prefix":"10.1128","volume":"75","author":[{"given":"Trevor K.","family":"Wagner","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha H.","family":"Mulks","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.4983-4987.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1139\/o86-097"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.11.3523-3530.1990"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.3.868-873.1994"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.24.8537-8541.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03442.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.58.3.466-490.1994"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02981.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08452.x"},{"key":"e_1_3_2_11_2","first-page":"209","volume":"7","year":"2005","unstructured":"Cowles, K. 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