{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T16:40:33Z","timestamp":1783960833387,"version":"3.55.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["877\/15"],"award-info":[{"award-number":["877\/15"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The nitrogen phosphotransferase system (PTS\n            <jats:sup>Ntr<\/jats:sup>\n            ) is a regulatory cascade present in many bacteria, where it controls different functions. This system is usually composed of three basic components: enzyme I\n            <jats:sup>Ntr<\/jats:sup>\n            (EI\n            <jats:sup>Ntr<\/jats:sup>\n            ), NPr, and EIIA\n            <jats:sup>Ntr<\/jats:sup>\n            (encoded by the\n            <jats:italic>ptsP<\/jats:italic>\n            ,\n            <jats:italic>ptsO<\/jats:italic>\n            , and\n            <jats:italic>ptsN<\/jats:italic>\n            genes, respectively). In\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            , as well as in many other\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            species, the EIIA\n            <jats:sup>Ntr<\/jats:sup>\n            component is missing. However, we found that deletion mutations in both\n            <jats:italic>ptsP<\/jats:italic>\n            and\n            <jats:italic>ptsO<\/jats:italic>\n            are partially attenuated for intracellular growth. Furthermore, these two PTS\n            <jats:sup>Ntr<\/jats:sup>\n            components were found to be required for maximal expression of effector-encoding genes regulated by the transcriptional activator PmrA. Genetic analyses which include the construction of single and double deletion mutants and overexpression of wild-type and mutated forms of EI\n            <jats:sup>Ntr<\/jats:sup>\n            , NPr, and PmrA indicated that the PTS\n            <jats:sup>Ntr<\/jats:sup>\n            components affect the expression of PmrA-regulated genes via PmrA and independently from PmrB and that EI\n            <jats:sup>Ntr<\/jats:sup>\n            and NPr are part of the same cascade and require their conserved histidine residues in order to function. Furthermore, expression of the\n            <jats:named-content content-type=\"genus-species\">Legionella micdadei<\/jats:named-content>\n            EII\n            <jats:sup>Ntr<\/jats:sup>\n            component in\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            resulted in a reduction in the levels of expression of PmrA-regulated genes which was completely dependent on the\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            PTS components and the\n            <jats:named-content content-type=\"genus-species\">L. micdadei<\/jats:named-content>\n            EII\n            <jats:sup>Ntr<\/jats:sup>\n            conserved histidine residue. Moreover, reconstruction of the\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            PTS\n            <jats:italic>in vitro<\/jats:italic>\n            indicated that EI\n            <jats:sup>Ntr<\/jats:sup>\n            is phosphorylated by phosphoenolpyruvate (PEP) and transfers its phosphate to NPr. Our results demonstrate that the\n            <jats:named-content content-type=\"genus-species\">L. pneumophila<\/jats:named-content>\n            incomplete PTS\n            <jats:sup>Ntr<\/jats:sup>\n            is functional and involved in the expression of effector-encoding genes regulated by PmrA.\n          <\/jats:p>","DOI":"10.1128\/iai.00121-17","type":"journal-article","created":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T00:39:29Z","timestamp":1491266369000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["The Legionella pneumophila Incomplete Phosphotransferase System Is Required for Optimal Intracellular Growth and Maximal Expression of PmrA-Regulated Effectors"],"prefix":"10.1128","volume":"85","author":[{"given":"Yariv","family":"Speiser","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology and Biotechnology, George S. 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