{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:21:05Z","timestamp":1790047265307,"version":"4.0.1"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Gram-negative bacterium\n            <jats:named-content content-type=\"genus-species\">Pasteurella multocida<\/jats:named-content>\n            is the causative agent of a number of economically important animal diseases, including avian fowl cholera. Numerous\n            <jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>\n            virulence factors have been identified, including capsule, lipopolysaccharide (LPS), and filamentous hemagglutinin, but little is known about how the expression of these virulence factors is regulated. Hfq is an RNA-binding protein that facilitates riboregulation via interaction with small noncoding RNA (sRNA) molecules and their mRNA targets. Here, we show that a\n            <jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>\n            <jats:italic>hfq<\/jats:italic>\n            mutant produces significantly less hyaluronic acid capsule during all growth phases and displays reduced\n            <jats:italic>in vivo<\/jats:italic>\n            fitness. Transcriptional and proteomic analyses of the\n            <jats:italic>hfq<\/jats:italic>\n            mutant during mid-exponential-phase growth revealed altered transcript levels for 128 genes and altered protein levels for 78 proteins. Further proteomic analyses of the\n            <jats:italic>hfq<\/jats:italic>\n            mutant during the early exponential growth phase identified 106 proteins that were produced at altered levels. Both the transcript and protein levels for genes\/proteins involved in capsule biosynthesis were reduced in the\n            <jats:italic>hfq<\/jats:italic>\n            mutant, as were the levels of the filamentous hemagglutinin protein PfhB2 and its secretion partner LspB2. In contrast, there were increased expression levels of three LPS biosynthesis genes, encoding proteins involved in phosphocholine and phosphoethanolamine addition to LPS, suggesting that these genes are negatively regulated by Hfq-dependent mechanisms. Taken together, these data provide the first evidence that Hfq plays a crucial role in regulating the global expression of\n            <jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>\n            genes, including the regulation of key\n            <jats:named-content content-type=\"genus-species\">P. multocida<\/jats:named-content>\n            virulence factors, capsule, LPS, and filamentous hemagglutinin.\n          <\/jats:p>","DOI":"10.1128\/iai.00122-16","type":"journal-article","created":{"date-parts":[[2016,2,17]],"date-time":"2016-02-17T03:58:22Z","timestamp":1455681502000},"page":"1361-1370","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["The RNA-Binding Chaperone Hfq Is an Important Global Regulator of Gene Expression in Pasteurella multocida and Plays a Crucial Role in Production of a Number of Virulence Factors, Including Hyaluronic Acid Capsule"],"prefix":"10.1128","volume":"84","author":[{"given":"Marianne","family":"M\u00e9groz","sequence":"first","affiliation":[{"name":"Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oded","family":"Kleifeld","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Powell","sequence":"additional","affiliation":[{"name":"Monash Bioinformatics Platform, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Harrison","sequence":"additional","affiliation":[{"name":"Monash Bioinformatics Platform, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Adler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Harper","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Boyce","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.02.005"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.06.009"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910308106"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07751.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032066599"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2012.664540"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2615"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a003798"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-111212-133445"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00436-1"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf322"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R112.441386"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.03.015"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg915"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04337.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.01.001"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00400.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01629.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05413.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006213"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01652-07"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01216-08"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04142.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3355-3362.2004"},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1002\/9780470958209.ch17","volume-title":"Pathogenesis of bacterial infections of animals","author":"Boyce JD","year":"2010","unstructured":"Boyce JD, Harper M, Wilkie IW, Adler B. 2010. Pasteurella, p 325\u2013346. In Gyles CL, Prescott JF, Songer JG, Thoen CO (ed), Pathogenesis of bacterial infections of animals, 4th ed. Blackwell Publishing, Ames, IA.","edition":"4"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079450050118395"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00024-13"},{"key":"e_1_3_3_29_2","first-page":"48","article-title":"The type specific capsular antigen of Pasteurella multocida","volume":"30","author":"Carter GR","year":"1952","unstructured":"Carter GR. 1952. The type specific capsular antigen of Pasteurella multocida. Can J Med Sci 30:48\u201353.","journal-title":"Can J Med Sci"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02824-14"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/82_2012_202"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2005.05.003"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11181.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/816713"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2487-2492.2001"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3463-3468.2000"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000750"},{"key":"e_1_3_3_38_2","volume-title":"Australian code of practice for the care and use of animals for scientific purposes","author":"National Medical Health and Research Council","year":"2004","unstructured":"National Medical Health and Research Council. 2004. Australian code of practice for the care and use of animals for scientific purposes, 7th ed. National Medical Health and Research Council, Canberra, Australia.","edition":"7"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00779-13"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.5440-5446.2003"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku536"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051634598"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1103"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1322"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.21"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00948-07"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01923-14"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004809"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02339.x"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(02)00702-5"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071933"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.4.1527-1532.2005"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05489.x"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-10"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-134"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022248"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01496-08"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00392-13"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2000.0365"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01144-08"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.05.022"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08017.x"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6871-6879.2002"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00187-X"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00122-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00122-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:20:06Z","timestamp":1646475606000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00122-16"}},"subtitle":[],"editor":[{"given":"G. H.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":66,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1128\/IAI.00122-16"],"URL":"https:\/\/doi.org\/10.1128\/iai.00122-16","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"2016-02-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}