{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:15:54Z","timestamp":1782918954374,"version":"3.54.5"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Macrophages (M\u03c6) may play an important role in the pathogenesis of invasive meningococcal infection. Previously, we have shown that the class A M\u03c6 scavenger receptor (SR-A) is a major nonopsonic receptor for<jats:italic>Neisseria meningitidis<\/jats:italic>on M\u03c6. SR-A contributes to host defense by binding proinflammatory polyanionic ligands such as lipopolysaccharide (LPS) and by the uptake and killing of live organisms. SR-A-deficient mouse M\u03c6 display a substantial reduction in the number of meningococci ingested compared to wild-type M\u03c6, and SR-A is required for meningococcal phagocytosis but not for the release of tumor necrosis factor alpha. Although soluble lipid A and lipid<jats:sub>IV<\/jats:sub>A are reported as ligands for SR-A, we demonstrated that LPS and LPS expression were not essential for the uptake of whole meningococci. In the present study, we set out to discover protein ligand(s) for SR-A in<jats:italic>N. meningitidis<\/jats:italic>lysates and outer membrane vesicles. Using various microbial mutant strains, we determined that molecules comprising the membrane capsule and pili, as well as the abundant surface Opa proteins were not essential for SR-A recognition. We developed a binding assay to detect SR-A ligands and identified three candidate proteins expressed on intact organisms, namely, NMB1220, NMB0278, and NMB0667. Soluble forms of these ligands were shown to block the binding of meningococci to CHO cells stably transfected with SR-A. Furthermore, NMB1220 was endocytosed by SR-A on M\u03c6 and prevented internalization of soluble acetylated low-density lipoprotein. Thus, we have identified novel, unmodified protein ligands for SR-A that are able to inhibit meningococcal interactions with macrophages in vitro.<\/jats:p>","DOI":"10.1128\/iai.00124-06","type":"journal-article","created":{"date-parts":[[2006,8,22]],"date-time":"2006-08-22T22:13:07Z","timestamp":1156284787000},"page":"5191-5199","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Identification of<i>Neisseria meningitidis<\/i>Nonlipopolysaccharide Ligands for Class A Macrophage Scavenger Receptor by Using a Novel Assay"],"prefix":"10.1128","volume":"74","author":[{"given":"Leanne","family":"Peiser","sequence":"first","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Makepeace","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Plu\u0308ddemann","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Savino","sequence":"additional","affiliation":[{"name":"IRIS, Chiron Vaccines, Via Fiorentina 1, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Claire","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariagrazia","family":"Pizza","sequence":"additional","affiliation":[{"name":"IRIS, Chiron Vaccines, Via Fiorentina 1, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rino","family":"Rappuoli","sequence":"additional","affiliation":[{"name":"IRIS, Chiron Vaccines, Via Fiorentina 1, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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