{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T21:05:04Z","timestamp":1767992704017,"version":"3.49.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The Toll-like receptors (TLRs) and the adaptor myeloid differentiation factor 88 (MyD88) are important in the innate immune defenses of the host to microbial infections. Meningococcal ligands signaling via TLRs control inflammatory responses, and stimulation can result in fulminant meningococcal sepsis. In this study, we show that the responses to nonlipooligosaccharide (non-LOS) ligands of meningococci are MyD88 dependent. An isogenic LOS-deficient mutant of the serogroup C meningococcal strain FAM20 caused fatal disease in wild type C57BL\/6 mice that was not observed in MyD88<jats:sup>\u2212\/\u2212<\/jats:sup>mice. Fatality correlated with high proinflammatory cytokine and C5a levels in serum, high neutrophil numbers in blood, and increased bacteremia at 24 h postinfection in the wild-type mice. Infection with the parent strain FAM20 resulted in fatality in 100% of the wild-type mice and 50% of the MyD88<jats:sup>\u2212\/\u2212<\/jats:sup>mice. We conclude that both LOS and another neisserial ligand cause meningococcal sepsis in an in vivo mouse model and confirm that meningococcal LOS can act via both the MyD88- dependent and -independent pathways, while the non-LOS meningococcal ligand(s) acts only via the MyD88-dependent pathway.<\/jats:p>","DOI":"10.1128\/iai.00128-06","type":"journal-article","created":{"date-parts":[[2006,5,19]],"date-time":"2006-05-19T23:52:41Z","timestamp":1148082761000},"page":"3538-3546","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["MyD88-Dependent Signaling Affects the Development of Meningococcal Sepsis by Nonlipooligosaccharide Ligands"],"prefix":"10.1128","volume":"74","author":[{"given":"Laura","family":"Plant","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Biomedical Centrum, Uppsala University, 751 23 Uppsala, Sweden"},{"name":"Smittskyddsinstitutet, Swedish Institute for Infectious Disease Control, Karolinska Institutet, Nobelsva\u0308g 18, 171 77 Solna, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Biomedical Centrum, Uppsala University, 751 23 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann-Beth","family":"Jonsson","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Biomedical Centrum, Uppsala University, 751 23 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.1.155-162.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519030090030301"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00519.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519010070060401"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115660"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.2.195"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/160.1.58"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/428590"},{"key":"e_1_3_2_10_2","first-page":"85","volume":"135","year":"2004","unstructured":"Dixon, G. L., R. S. Heyderman, P. van der Ley, and N. J. Klein. 2004. High-level endothelial E-selectin (CD62E) cell adhesion molecule expression by a lipopolysaccharide-deficient strain of Neisseria meningitidis despite poor activation of NF-kappaB transcription factor. Clin. Exp. Immunol.135:85-93.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4351-4357.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.7.3195"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(87)90005-2"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306587200"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.4049\/jimmunol.153.6.2618","volume":"153","year":"1994","unstructured":"Florquin, S., Z. Amraoui, D. Abramowicz, and M. Goldman. 1994. Systemic release and protective role of IL-10 in staphylococcal enterotoxin B-induced shock in mice. J. Immunol.153:2618-2623.","journal-title":"J. Immunol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115835"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2004.00471.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519000060050301"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408419891294216"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.10.5887"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.85.6.1274-1279.1963"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.1.438"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6917-6923.2000"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01540884"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.4.1533"},{"key":"e_1_3_2_26_2","first-page":"BR316","volume":"9","year":"2003","unstructured":"Mirlashari, M. R., and T. Lyberg. 2003. Expression and involvement of Toll-like receptors (TLR)2, TLR4, and CD14 in monocyte TNF-alpha production induced by lipopolysaccharides from Neisseria meningitidis. Med. Sci. Monit.9:BR316-BR324.","journal-title":"Med. Sci. Monit."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.001121"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.153.5.1302"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.1.450"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503225200"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.320.7238.846"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5346-5354.2002"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1086\/317647"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4601823.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.8.2.8119492"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.6.3545"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1159\/000236329"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-03-0703"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.06.007"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3344-3349.2004"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1189\/jlb.70.2.283","volume":"70","year":"2001","unstructured":"Sprong, T., N. Stikkelbroeck, P. van der Ley, L. Steeghs, L. van Alphen, N. Klein, M. G. Netea, J. W. van der Meer, and M. van Deuren. 2001. Contributions of Neisseria meningitidis LPS and non-LPS to proinflammatory cytokine response. J. Leukoc. Biol.70:283-288.","journal-title":"J. Leukoc. Biol."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2003.10.003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115843"},{"key":"e_1_3_2_44_2","first-page":"312","volume":"122","year":"2000","unstructured":"Uronen, H., A. J. Williams, G. Dixon, S. R. Andersen, P. Van Der Ley, M. Van Deuren, R. E. Callard, and N. Klein. 2000. Gram-negative bacteria induce proinflammatory cytokine production by monocytes in the absence of lipopolysaccharide (LPS). Clin. Exp. Immunol.122:312-315.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519030090020901"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.9.6042"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.1.371-380.2004"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.2940-2950.2005"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00128-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00128-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,7]],"date-time":"2023-05-07T13:16:46Z","timestamp":1683465406000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00128-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1128\/IAI.00128-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.00128-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6]]},"assertion":[{"value":"2006-01-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}