{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:19:01Z","timestamp":1763036341890},"reference-count":33,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Tuberculous pleurisy is a frequent extrapulmonary manifestation characterized by accumulation of fluid and inflammatory cells in the pleural space. Here, we investigated the mechanisms of T-lymphocyte accumulation in the pleural space by using a murine model of pleurisy induced by<jats:italic>Mycobacterium bovis<\/jats:italic>BCG. Intrathoracic (i.t.) injection of BCG (4.5 \u00d7 10<jats:sup>5<\/jats:sup>bacteria\/cavity) induced accumulation of T lymphocytes in the pleural cavities of C57BL\/6 mice. We observed the presence of CFU in pleural washes conducted 1, 2, 3, 7, and 15 days after pleurisy induction. Pretreatment with fucoidan inhibited T-lymphocyte accumulation at 1 day, but not at 15 days, after BCG-induced pleurisy. Accordingly, adoptive transfer of fluorescein isothiocyanate-labeled blood mononuclear cells to infected mice showed that T lymphocytes migrated into the pleural cavity 1 day (but not 15 days) after BCG injection. Cell-free pleural wash fluids recovered from mice 1 day after BCG i.t. stimulation (day 1 BCG-PW), but not day 7 or day 15 BCG-PW, induced in vitro T-cell transmigration, which was dependent on L-, P-, and E-selectins. In contrast, day 7 BCG-PW (but not day 1 BCG-PW) induced in vitro T-lymphocyte proliferation via interleukin-2 (IL-2) and gamma interferon (IFN-\u03b3). Accordingly, in vivo IL-2 or IFN-\u03b3 neutralization abolished T-lymphocyte accumulation 7 days after pleurisy induction. Our results demonstrate that pleural infection induced by BCG leads to T-lymphocyte accumulation in two waves. The acute phase depends on selectin-mediated migration, while the second wave of T-lymphocyte accumulation seems to depend on a local proliferation induced by cytokines produced in situ.<\/jats:p>","DOI":"10.1128\/iai.00133-08","type":"journal-article","created":{"date-parts":[[2008,9,23]],"date-time":"2008-09-23T00:25:42Z","timestamp":1222129542000},"page":"5686-5693","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Mechanisms of T-Lymphocyte Accumulation during Experimental Pleural Infection Induced by<i>Mycobacterium bovis<\/i>BCG"],"prefix":"10.1128","volume":"76","author":[{"given":"Mariana C.","family":"Souza","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Farmacologia Aplicada, Departamento de Farmacologia Aplicada, Farmanguinhos, Fundac\u0327a\u0303o Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Penido","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Farmacologia Aplicada, Departamento de Farmacologia Aplicada, Farmanguinhos, Fundac\u0327a\u0303o Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria F. S.","family":"Costa","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Farmacologia Aplicada, Departamento de Farmacologia Aplicada, Farmanguinhos, Fundac\u0327a\u0303o Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grac\u0327as","family":"Henriques","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Farmacologia Aplicada, Departamento de Farmacologia Aplicada, Farmanguinhos, Fundac\u0327a\u0303o Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.12.1054"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4271-4277.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.5.1653"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00403902"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(97)00072-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.91.3.338"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1189\/jlb.70.6.950","volume":"70","year":"2001","unstructured":"Bernabei, P., E. M. Coccia, L. Rigamonti, M. Bosticardo, G. Forni, S. Pestka, C. D. Krause, A. Battistini, and F. Novelli. 2001. Interferon-gamma receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death. J. Leukoc. Biol.70:950-960.","journal-title":"J. Leukoc. Biol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.12.6711"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2004.01.006"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.1.463"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.12.1711"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2003.00539.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00303-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.97.10102411"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1351"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761997000800033"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.62.6.778"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00408-003-1006-1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.159.5.9810011"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050465"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/315057"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.4049\/jimmunol.157.5.1935","volume":"157","year":"1996","unstructured":"Novelli, F., P. Bernabei, L. Ozmen, L. Rigamonti, A. Allione, S. Pestka, G. Garotta, and G. Forni. 1996. Switching on of the proliferation or apoptosis of activated human T lymphocytes by IFN-gamma is correlated with the differential expression of the alpha- and beta-chains of its receptor. J. Immunol.157:1935-1943.","journal-title":"J. Immunol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.12.6788"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20252"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.2.1131"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"354","DOI":"10.4049\/jimmunol.132.1.354","volume":"132","year":"1984","unstructured":"Spangrude, G. J., B. A. Braaten, and R. A. Daynes. 1984. Molecular mechanisms of lymphocyte extravasation. I. Studies of two selective inhibitors of lymphocyte recirculation. J. Immunol.132:354-362.","journal-title":"J. Immunol."},{"key":"e_1_3_2_28_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"963","DOI":"10.4049\/jimmunol.161.2.963","volume":"161","year":"1998","unstructured":"Tietz, W., Y. Allemand, E. Borges, D. von Laer, R. Hallmann, D. Vestweber, and A. Hamann. 1998. CD41 T cells migrate into inflamed skin only if they express ligands for E- and P-selectin1. J. Immunol.161:963-970.","journal-title":"J. Immunol."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.1.317-326.2003"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050581"},{"key":"e_1_3_2_32_2","unstructured":"Culture. 1998"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5537-5542.1998"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.20.13744"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00133-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00133-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T01:47:22Z","timestamp":1684547242000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00133-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["10.1128\/IAI.00133-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.00133-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]},"assertion":[{"value":"2008-01-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-09-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}