{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:21:15Z","timestamp":1789730475384,"version":"4.0.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["R01-AI125489"],"award-info":[{"award-number":["R01-AI125489"]}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["PARTID18P0"],"award-info":[{"award-number":["PARTID18P0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2024,8,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      \u03b1-hemolysin (Hla) is a pore-forming toxin critical for the pathogenesis of skin and soft tissue infections, which causes the pathognomonic lesion of cutaneous necrosis (dermonecrosis) in mouse models. To determine the mechanism by which dermonecrosis develops during\n                      <jats:italic>S. aureus<\/jats:italic>\n                      skin infection, mice were given control serum, Hla-neutralizing antiserum, or an inhibitor of Hla receptor [A-disintegrin and metalloprotease 10 (ADAM10) inhibitor] followed by subcutaneous infection by\n                      <jats:italic>S. aureus,<\/jats:italic>\n                      and the lesions were evaluated using immunohistochemistry and immunofluorescence. Hla induced apoptosis in the vascular endothelium at 6 hours post-infection (hpi), followed by apoptosis in keratinocytes at 24 hpi. The loss of vascular endothelial (VE)-cadherin expression preceded the loss of epithelial-cadherin expression. Hla also induced hypoxia in the keratinocytes at 24 hpi following vascular injury. Treatment with Hla-neutralizing antibody or ADAM10 inhibitor attenuated early cleavage of VE-cadherin, cutaneous hypoxia, and dermonecrosis. These findings suggest that Hla-mediated vascular injury with cutaneous hypoxia underlies the pathogenesis of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      -induced dermonecrosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00133-24","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T09:02:17Z","timestamp":1719910937000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["\u03b1-Hemolysin-mediated endothelial injury contributes to the development of\n                    <i>Staphylococcus aureus<\/i>\n                    -induced dermonecrosis"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5497-8616","authenticated-orcid":false,"given":"Ching","family":"Yang","sequence":"first","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital , Columbus, Ohio, USA"},{"name":"Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University , Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5583-9194","authenticated-orcid":false,"given":"Frank H.","family":"Robledo-Avila","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital , Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2996-9440","authenticated-orcid":false,"given":"Santiago","family":"Partida-Sanchez","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital , Columbus, Ohio, USA"},{"name":"Department of Pediatrics, College of Medicine, The Ohio State University 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