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In polarized epithelial cells, efficient protrusion formation by\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              requires the secreted bacterial protein InlC, which binds to a carboxyl-terminal Src homology 3 (SH3) domain in the human scaffolding protein Tuba. This interaction antagonizes Tuba, thereby diminishing cortical tension at the apical junctional complex and enhancing\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              protrusion formation and spread. Tuba contains five SH3 domains apart from the domain that interacts with InlC. Here, we show that human GTPase Dynamin 2 associates with two SH3 domains in the amino-terminus of Tuba and acts together with this scaffolding protein to control the spread of\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              . Genetic or pharmacological inhibition of Dynamin 2 or knockdown of Tuba each restored normal protrusion formation and spread to a bacterial strain deleted for the\n              <jats:italic>inlC<\/jats:italic>\n              gene (\u2206\n              <jats:italic>inlC<\/jats:italic>\n              ). Dynamin 2 localized to apical junctions in uninfected human cells and protrusions in cells infected with\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              . Localization of Dynamin 2 to junctions and protrusions depended on Tuba. Knockdown of Dynamin 2 or Tuba diminished junctional linearity, indicating a role for these proteins in controlling cortical tension. Infection with\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              induced InlC-dependent displacement of Dynamin 2 from junctions, suggesting a possible mechanism of antagonism of this GTPase. Collectively, our results show that Dynamin 2 cooperates with Tuba to promote intercellular tension that restricts the spread of \u2206\n              <jats:italic>inlC Listeria<\/jats:italic>\n              . By expressing InlC, wild-type\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              overcomes this restriction.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00136-24","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T13:02:04Z","timestamp":1723467724000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["The host GTPase Dynamin 2 modulates apical junction structure to control cell-to-cell spread of\n            <i>Listeria monocytogenes<\/i>"],"prefix":"10.1128","volume":"92","author":[{"given":"Serena","family":"Tijoriwalla","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiloma","family":"Liyanage","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thilina U. 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