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Here, we describe the bioinformatic analysis of the family of 18 predicted CWA proteins encoded in the genome of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. pseudintermedius<\/jats:named-content>strain ED99 and determine their distribution among a phylogenetically diverse panel of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. pseudintermedius<\/jats:named-content>clinical isolates and closely related species of the<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus intermedius<\/jats:named-content>group. In parallel, we employed a proteomic approach to identify proteins presented on the surface of strain ED99<jats:italic>in vitro<\/jats:italic>, revealing a total of 60 surface-localized proteins in one or more phases of growth, including 6 of the 18 genome-predicted CWA proteins. Based on these analyses, we selected two CWA proteins (SpsD and SpsL) encoded by all strains examined and investigated their capacity to mediate adherence to extracellular matrix proteins. We discovered that SpsD and SpsL mediated binding of a heterologous host,<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Lactococcus lactis<\/jats:named-content>, to fibrinogen and fibronectin and that SpsD mediated binding to cytokeratin 10, a major constituent of mammalian skin. Of note, the interaction with fibrinogen was host-species dependent, suggestive of a role for SpsD and SpsL in the host tropism of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. pseudintermedius<\/jats:named-content>. Finally, we identified IgG specific for SpsD and SpsL in sera from dogs with bacterial pyoderma, implying that both proteins are expressed during infection. The combined genomic and proteomic approach employed in the current study has revealed novel host-pathogen interactions which represent candidate therapeutic targets for the control of bacterial pyoderma.<\/jats:p>","DOI":"10.1128\/iai.00137-11","type":"journal-article","created":{"date-parts":[[2011,5,17]],"date-time":"2011-05-17T01:57:30Z","timestamp":1305597450000},"page":"3074-3086","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Genomic and Surface Proteomic Analysis of the Canine Pathogen Staphylococcus pseudintermedius Reveals Proteins That Mediate Adherence to the Extracellular Matrix"],"prefix":"10.1128","volume":"79","author":[{"given":"Jeanette","family":"Bannoehr","sequence":"first","affiliation":[{"name":"The Roslin Institute and Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom"},{"name":"Dermatology Unit, Division of Veterinary Clinical Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouri L.","family":"Ben Zakour","sequence":"additional","affiliation":[{"name":"The Roslin Institute and Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Reglinski","sequence":"additional","affiliation":[{"name":"The Roslin Institute and Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil F.","family":"Inglis","sequence":"additional","affiliation":[{"name":"Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabitha","family":"Prabhakaran","sequence":"additional","affiliation":[{"name":"Center for Infectious and Inflammatory Diseases, Institute of Biosciences and Technology, Texas A&amp;M University System Health Science Center, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Even","family":"Fossum","sequence":"additional","affiliation":[{"name":"Division of Pathway Medicine, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Smith","sequence":"additional","affiliation":[{"name":"Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, United Kingdom"},{"name":"Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G61 1QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian J.","family":"Wilson","sequence":"additional","affiliation":[{"name":"The Roslin Institute and Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robyn A.","family":"Cartwright","sequence":"additional","affiliation":[{"name":"The Roslin Institute and Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Haas","sequence":"additional","affiliation":[{"name":"Division of Pathway Medicine, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Hook","sequence":"additional","affiliation":[{"name":"Center for Infectious and Inflammatory Diseases, Institute of Biosciences and Technology, Texas A&amp;M University System Health Science Center, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adri H. 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