{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:43:48Z","timestamp":1760121828960,"version":"3.37.3"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Bordetella avium<\/jats:italic>causes bordetellosis in birds, a disease similar to whooping cough caused by<jats:italic>Bordetella pertussis<\/jats:italic>in children.<jats:italic>B. avium<\/jats:italic>agglutinates guinea pig erythrocytes via an unknown mechanism. Loss of hemagglutination ability results in attenuation. We report the use of transposon mutagenesis to identify two genes required for hemagglutination. The genes (<jats:italic>hagA<\/jats:italic>and<jats:italic>hagB<\/jats:italic>) were adjacent and divergently oriented and had no orthologs in the genomes of other<jats:italic>Bordetella<\/jats:italic>species. Construction of in-frame, unmarked mutations in each gene allowed examination of the role of each in conferring erythrocyte agglutination, explanted tracheal cell adherence, and turkey poult tracheal colonization. In all of the<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>assays, the requirement for the<jats:italic>trans<\/jats:italic>-acting products of<jats:italic>hagA<\/jats:italic>and<jats:italic>hagB<\/jats:italic>(HagA and HagB) was readily shown. Western blotting, using antibodies to purified HagA and HagB, revealed proteins of the predicted sizes of HagA and HagB in an outer membrane-enriched fraction. Antiserum to HagB, but not HagA, blocked<jats:italic>B. avium<\/jats:italic>erythrocyte agglutination and explanted turkey tracheal ring binding. Bioinformatic analysis indicated the similarity of HagA and HagB to several two-component secretory apparatuses in which one product facilitates the exposition of the other. HagB has the potential to serve as a useful immunogen to protect turkeys against colonization and subsequent disease.<\/jats:p>","DOI":"10.1128\/iai.00140-10","type":"journal-article","created":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T03:28:48Z","timestamp":1269919728000},"page":"2370-2376","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Identification and Characterization of Two<i>Bordetella avium<\/i>Gene Products Required for Hemagglutination"],"prefix":"10.1128","volume":"78","author":[{"given":"Louise M.","family":"Temple","sequence":"first","affiliation":[{"name":"James Madison University, Harrisonburg, Virginia 22807"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Drew University, Madison, New Jersey 07940"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manju","family":"Mehta","sequence":"additional","affiliation":[{"name":"Drew University, Madison, New Jersey 07940"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian M.","family":"Capitini","sequence":"additional","affiliation":[{"name":"Drew University, Madison, New Jersey 07940"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Von Stetina","sequence":"additional","affiliation":[{"name":"Drew University, Madison, New Jersey 07940"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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