{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T01:28:40Z","timestamp":1773192520668,"version":"3.50.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The impact of the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Borrelia burgdorferi<\/jats:named-content>\n            surface-localized immunogenic lipoprotein BBA66 on vector and host infection was evaluated by inactivating the encoding gene,\n            <jats:italic>bba66<\/jats:italic>\n            , and characterizing the mutant phenotype throughout the natural mouse-tick-mouse cycle. The BBA66-deficient mutant isolate, Bb\n            <jats:sup>\u0394A66<\/jats:sup>\n            , remained infectious in mice by needle inoculation of cultured organisms, but differences in spirochete burden and pathology in the tibiotarsal joint were observed relative to the parental wild-type (WT) strain.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Ixodes scapularis<\/jats:named-content>\n            larvae successfully acquired Bb\n            <jats:sup>\u0394A66<\/jats:sup>\n            following feeding on infected mice, and the organisms persisted in these ticks through the molt to nymphs. A series of tick transmission experiments (\n            <jats:italic>n<\/jats:italic>\n            = 7) demonstrated that the ability of Bb\n            <jats:sup>\u0394A66<\/jats:sup>\n            -infected nymphs to infect laboratory mice was significantly impaired compared to that of mice fed upon by WT-infected ticks.\n            <jats:italic>trans<\/jats:italic>\n            -complementation of Bb\n            <jats:sup>\u0394A66<\/jats:sup>\n            with an intact copy of\n            <jats:italic>bba66<\/jats:italic>\n            restored the WT infectious phenotype in mice via tick transmission. These results suggest a role for BBA66 in facilitating\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            dissemination and transmission from the tick vector to the mammalian host as part of the disease process for Lyme borreliosis.\n          <\/jats:p>","DOI":"10.1128\/iai.00140-13","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T04:03:46Z","timestamp":1367294626000},"page":"2488-2498","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["<i>Borrelia burgdorferi bba66<\/i>\n            Gene Inactivation Results in Attenuated Mouse Infection by Tick Transmission"],"prefix":"10.1128","volume":"81","author":[{"given":"Toni G.","family":"Patton","sequence":"first","affiliation":[{"name":"National Center for Emerging and Zoonotic Infectious 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