{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T13:22:57Z","timestamp":1793366577406,"version":"4.5.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,5,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Cryptococcal meningitis is a common and refractory central nervous system (CNS) infection with high mortality and disability. For\n              <jats:italic toggle=\"yes\">Cryptococcus neoformans<\/jats:italic>\n              (\n              <jats:italic toggle=\"yes\">C. neoformans<\/jats:italic>\n              ) to penetrate the CNS, it first adheres to and breaches the blood\u2012brain barrier (BBB). Here, we explored the roles of CD146, an adhesion molecule expressed on the surface of brain microvascular endothelial cells (BMECs), in cryptococcal vascular adhesion and BBB invasion. Following cryptococcal infection, we observed a reduction in CD146 expression in BMECs, which was at least partially mediated by metalloproteinase-9. Once overexpressed on BMECs, CD146 increased\n              <jats:italic toggle=\"yes\">C. neoformans<\/jats:italic>\n              adhesion; in contrast, CD146 knockout decreased the attachment of fungi to endothelial cells\n              <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n              . Unexpectedly, CD146 knockout failed to reduce fungal infection in the brain following intravascular instillation of\n              <jats:italic toggle=\"yes\">C. neoformans<\/jats:italic>\n              . However,\n              <jats:italic toggle=\"yes\">the<\/jats:italic>\n              anti-CD146 antibody AA98 significantly increased the fungemia (spleen CFU), suggesting that CD146 may be involved in the early adhesion and invasion of Cryptococcus into cerebral vessels. AA98, however, failed to extend the survival of\n              <jats:italic toggle=\"yes\">C. neoformans<\/jats:italic>\n              infected mice. These results suggest that CD146 may play dispensable roles in the\n              <jats:italic toggle=\"yes\">C. neoformans<\/jats:italic>\n              brain infection.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00145-25","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T13:54:46Z","timestamp":1744293286000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Involvement of CD146 in the\n            <i>Cryptococcus neoformans<\/i>\n            adhesion and infection of brain endothelial cells"],"prefix":"10.1128","volume":"93","author":[{"given":"Wei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Immunology, National Vaccine Innovation Platform, NHC Key Laboratory of Antibody Technique, Jiangsu Key Laboratory of Pathogen Biology, School of Basic Medical Sciences, Nanjing Medical University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Immunology, National Vaccine Innovation Platform, NHC Key Laboratory of Antibody Technique, Jiangsu Key Laboratory of Pathogen Biology, School of Basic Medical Sciences, Nanjing Medical University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Immunology, National Vaccine Innovation Platform, NHC Key Laboratory of Antibody Technique, Jiangsu Key Laboratory of Pathogen Biology, School of Basic Medical Sciences, Nanjing Medical University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Immunology, National Vaccine Innovation Platform, NHC Key Laboratory of Antibody Technique, 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China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyun","family":"Yan","sequence":"additional","affiliation":[{"name":"Nanozyme Laboratory in Zhongyuan, Henan Academy of Innovations in Medical Science","place":["Zhengzhou, China"]},{"name":"CAS Engineering Laboratory for Nanozyme, Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3995-1228","authenticated-orcid":false,"given":"Hongxia","family":"Duan","sequence":"additional","affiliation":[{"name":"Nanozyme Laboratory in Zhongyuan, Henan Academy of Innovations in Medical Science","place":["Zhengzhou, China"]},{"name":"CAS Engineering Laboratory for Nanozyme, Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences","place":["Beijing, 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