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The high-temperature requirement A (HtrA)-like protease, which is involved in protein quality control, has been reported to be a virulence factor in many pathogens. In this study, we showed that HtrA of\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            (HpHtrA) exhibited both chaperone and protease activities. Finally, nickel import ATP-binding protein (NikE), periplasmic dipeptide transport protein (DppA), and outer membrane protein A (OmpA) were identified as proteolytic substrates for HpHtrA. The protease activity reached its maximum at 40\u00b0C in a time-dependent manner. Disruption of the\n            <jats:italic>htrA<\/jats:italic>\n            gene from strain SC1401 affected tolerance to temperature stress and resistance to complement-mediated killing. Furthermore, increased autoagglutination and biofilm formation were detected in the\n            <jats:italic>htrA<\/jats:italic>\n            mutant. In addition, the\n            <jats:italic>htrA<\/jats:italic>\n            mutant was significantly attenuated in virulence in the murine model of infection. Together, these data demonstrate that HpHtrA plays an important role in the virulence of\n            <jats:named-content content-type=\"genus-species\">H. parasuis<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00147-16","type":"journal-article","created":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T03:23:29Z","timestamp":1464060209000},"page":"2209-2219","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["HtrA Is Important for Stress Resistance and Virulence in Haemophilus parasuis"],"prefix":"10.1128","volume":"84","author":[{"given":"Luhua","family":"Zhang","sequence":"first","affiliation":[{"name":"Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center of 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