{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T15:26:07Z","timestamp":1770996367125,"version":"3.50.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antibody (Ab) responses to\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            toxins are protective, but relatively few protective monoclonal antibodies (MAbs) have been reported. Protective antigen (PA) is essential for the action of\n            <jats:italic>B. anthracis<\/jats:italic>\n            lethal toxin (LeTx) and edema toxin. In this study, we generated two MAbs to PA, MAbs 7.5G and 10F4. These MAbs did not compete for binding to PA, consistent with specificities for different epitopes. The MAbs were tested for their ability to protect a monolayer of cultured macrophages against toxin-mediated cytotoxicity. MAb 7.5G, the most-neutralizing MAb, bound to domain 1 of PA and reduced LeTx toxicity in BALB\/c mice. Remarkably, MAb 7.5G provided protection without blocking the binding of PA or lethal factor or the formation of the PA heptamer complex. However, MAb 7.5G slowed the proteolytic digestion of PA by furin in vitro, suggesting a potential mechanism for Ab-mediated protection. These observations indicate that some Abs to domain 1 can contribute to host protection.\n          <\/jats:p>","DOI":"10.1128\/iai.00150-06","type":"journal-article","created":{"date-parts":[[2006,6,21]],"date-time":"2006-06-21T23:10:29Z","timestamp":1150931429000},"page":"4149-4156","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["A Monoclonal Antibody to\n            <i>Bacillus anthracis<\/i>\n            Protective Antigen Defines a Neutralizing Epitope in Domain 1"],"prefix":"10.1128","volume":"74","author":[{"given":"Johanna","family":"Rivera","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Nakouzi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nareen","family":"Abboud","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina","family":"Revskaya","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Goldman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. John","family":"Collier","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina","family":"Dadachova","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"},{"name":"Department of Nuclear Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arturo","family":"Casadevall","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"},{"name":"Department of Medicine, Division of Infectious Diseases"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.7.2209"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.11.6313-6317.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(92)90209-C"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.111301.140655"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.7006-7010.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8051(97)00079-6"},{"key":"e_1_3_2_8_2","first-page":"1","volume":"47","year":"1988","unstructured":"DeLorme, M. 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