{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T19:37:45Z","timestamp":1793129865803,"version":"4.5.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["F30 DK138707"],"award-info":[{"award-number":["F30 DK138707"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["P30-DK117469"],"award-info":[{"award-number":["P30-DK117469"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of General Medical Sciences of the National Institutes of Health","award":["P20-GM113132"],"award-info":[{"award-number":["P20-GM113132"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Institute of Allergy and Infectious Diseases of the National Institutes of Health","award":["R01 AI168411"],"award-info":[{"award-number":["R01 AI168411"]}],"id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Institute of General Medical Sciences of the National Institutes of Health","award":["T32 GM152319"],"award-info":[{"award-number":["T32 GM152319"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2026,9,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Innate immune signaling plays a key role in host response to infection, yet the pattern recognition receptors that detect non-model gut-associated yeasts remain poorly defined. Here, we investigated macrophage sensing of\n                      <jats:italic toggle=\"yes\">Debaryomyces hansenii<\/jats:italic>\n                      , a food-derived yeast that we found to be enriched within intestinal ulcers of Crohn disease (CD) patients. Using a cell surface receptor antibody screen of bone marrow-derived macrophages infected with a CD patient isolate of\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      , we showed that\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      -induced macrophage activation characterized by increased expression of co-stimulatory molecules, MHC-II, and pattern recognition receptors, including the C-type lectin receptor Dectin-1. Antibody blockade experiments showed both Dectin-1 and complement receptor 3 subunit CD11b were required for phagocytosis of\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      , while Dectin-1 was uniquely required for production of the pro-inflammatory cytokine tumor necrosis factor (Tnf). CRISPR-Cas9-mediated deletion of Dectin-1 phenocopied antibody neutralization effects on phagocytosis. Furthermore, deletion of Dectin-1 or its downstream signaling adaptor molecule Card9 resulted in reduced Tnf secretion in response to\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      . Dectin-1-mediated uptake of\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      was observed in primary bone marrow-derived macrophage and dendritic cells, as well as across the spectrum of macrophage polarization states. Together, these findings define the role of Dectin-1-Card9 signaling axis in innate immune cell sensing of\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      . These findings support the emerging relevance of innate immune recognition of a yeast in Crohn disease pathogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00157-26","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:56:26Z","timestamp":1785851786000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Innate immune recognition of\n                    <i>Debaryomyces hansenii<\/i>\n                    requires Dectin-1-Card9 signaling"],"prefix":"10.1128","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4226-718X","authenticated-orcid":true,"given":"Kevin P.","family":"Newhall","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xjacd83","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Inflammation and Immunity, Cleveland Clinic Research","place":["Cleveland, USA"]},{"id":[{"id":"https:\/\/ror.org\/02x4b0932","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology, Case Western Reserve University School of Medicine","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3469-3231","authenticated-orcid":true,"given":"Sarah K.","family":"McNeer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xjacd83","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Inflammation and Immunity, Cleveland Clinic Research","place":["Cleveland, USA"]},{"id":[{"id":"https:\/\/ror.org\/02x4b0932","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology, Case Western Reserve University School of Medicine","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8885-7532","authenticated-orcid":true,"given":"Scott T.","family":"Espenschied","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xjacd83","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Inflammation and Immunity, Cleveland Clinic Research","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1709-7430","authenticated-orcid":true,"given":"Elora G.","family":"Dolan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1533-5183","authenticated-orcid":true,"given":"Julie Y.","family":"Zhou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xjacd83","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Inflammation and Immunity, Cleveland Clinic Research","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6366-2971","authenticated-orcid":true,"given":"Deborah A.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6023-3901","authenticated-orcid":true,"given":"Thaddeus S.","family":"Stappenbeck","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xjacd83","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Inflammation and Immunity, Cleveland Clinic Research","place":["Cleveland, USA"]},{"name":"Cleveland Clinic Lerner College of Medicine, Case Western Reserve University","place":["Cleveland, 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KP Hogan DA Stappenbeck TS. 2026. Innate immune recognition of Debaryomyces hansenii requires Dectin-1-Card9 signaling. Infection and Immunity. 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