{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:09:23Z","timestamp":1793660963011,"version":"4.5.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002156"],"award-info":[{"award-number":["82002156"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302568"],"award-info":[{"award-number":["82302568"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Anhui Provincial Department of Education","award":["2024AH050743"],"award-info":[{"award-number":["2024AH050743"]}]},{"DOI":"10.13039\/501100022650","name":"Anhui Postdoctoral Science Foundation","doi-asserted-by":"crossref","award":["2308085QH283"],"award-info":[{"award-number":["2308085QH283"]}],"id":[{"id":"10.13039\/501100022650","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,8,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Spotted fever group\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      (SFGR) poses a significant challenge in the field of tick-borne diseases, characterized by its obligate intracellular lifestyle and its ability to disrupt various host cellular pathways. A deeper understanding of\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      \u2019s interactions with immune signaling is crucial for the development of novel therapeutic strategies. While previous research has shown that SFGR infection manipulates host cell death responses, the specific effects on receptor-interacting protein kinase 1 (RIPK1)-mediated multiple cell death pathways\u2014collectively referred to as PANoptosis\u2014remain poorly understood. In this study, we reveal that infection with\n                      <jats:italic toggle=\"yes\">Rickettsia heilongjiangensis<\/jats:italic>\n                      suppresses RIPK1 kinase-dependent apoptosis and necroptosis in human microvascular endothelial cells (HMEC-1). However, mitochondria-dependent apoptosis during the late stages of infection is essential for bacterial replication. Interestingly, inhibition of caspase-8 does not sensitize\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      -infected host cells to necroptosis. Transcriptomic analysis further reveals that\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      infection upregulates the host tumor necrosis factor (TNF) and NF-\u03baB signaling pathways, which subsequently suppress RIPK1 kinase activity and contribute to the inhibition of host cell death. These findings provide new insights into the molecular mechanisms by which\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      evades host defenses.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00158-25","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T10:00:57Z","timestamp":1751536857000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["<i>Rickettsia heilongjiangensis<\/i>\n                    suppresses RIPK1 kinase-mediated host cell death during the infection"],"prefix":"10.1128","volume":"93","author":[{"given":"Maozhang","family":"He","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xb04968","id-type":"ROR","asserted-by":"publisher"}],"name":"Anhui Province Key Laboratory of Zoonoses, Department of Microbiology, School of Basic Medical Sciences, Anhui 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