{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T13:03:34Z","timestamp":1793192614887,"version":"4.5.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["T32 OD011083"],"award-info":[{"award-number":["T32 OD011083"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 A1166162"],"award-info":[{"award-number":["R21 A1166162"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01 DK114224"],"award-info":[{"award-number":["R01 DK114224"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2026,6,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Diabetes and urinary tract infection (UTI) are major global public health problems. We investigated the effects of glucose on bacterial colonization and host response to UTI caused by uropathogenic\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      (UPEC) in a mouse model of type 2 diabetes mellitus (T2DM) of polygenic origin. Dapagliflozin was used to induce hyperglucosuria in diabetic and healthy adult mice before and during experimental UTI with UPEC. Glucose, bacterial load, cytokines, tissue damage scores, and myeloid cell composition during acute UTI were determined. A UPEC mutant lacking a gluconeogenesis pathway was constructed to test the role of glucose on pathogen fitness in human urine and during UTI in diabetic mice. A significant increase in UPEC load was observed in the urinary tract of hyperglucosuric diabetic mice with a higher incidence of systemic dissemination and a higher degree of cystitis and bladder neutrophil counts compared to vehicle-treated controls. We also observed higher bacterial titer in vehicle-treated diabetic mice compared to healthy mice. While there was a significant increase in tissue damage in diabetic, hyperglucosuric mice, the levels of TNF-\u03b1, IL-1\u03b2, and IL-6 in the urinary tract were similar across treatment groups and genotypes. Our\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      competition experiments highlight the importance of the gluconeogenesis pathway in UPEC for optimal fitness of the pathogen in both healthy and diabetic hosts. Collectively, our results indicate that both hyperglucosuria and T2DM promote the onset of severe UTI, and systemic dissemination of urinary tract origin is likely caused by altered neutrophil trafficking to the urinary bladder in a mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00172-26","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:07:22Z","timestamp":1777572442000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Diabetes and hyperglucosuria exacerbate the severity of urinary tract infection caused by uropathogenic\n                    <i>E<\/i>\n                    .\n                    <i>coli<\/i>\n                    in the mouse model"],"prefix":"10.1128","volume":"94","author":[{"given":"Sarita","family":"Neupane","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"George","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veerakit","family":"Vanitshavit","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessa","family":"Hejtmancik","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da Mi","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gus","family":"Wright","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Garry","family":"Adams","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1217-9813","authenticated-orcid":true,"given":"Sargurunathan","family":"Subashchandrabose","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3432"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2013.09.003"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/318850"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1104429"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-93912-3"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.UTI-0015-2012"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2012.00339.x"},{"key":"e_1_3_3_9_2","unstructured":"Anonymous. 2024. 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