{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:42:06Z","timestamp":1765546926529,"version":"3.41.2"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Translational Research Program, Boston Children's Hospital","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016270","name":"Global Challenges Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2022,7,21]]},"abstract":"<jats:p>The advent of pneumococcal conjugate vaccines led to the near disappearance of most of the included serotypes in high-income settings but also the rise of nonvaccine-type colonization and disease. Alternative strategies, using genetically conserved proteins as antigens, have been evaluated preclinically and clinically for years, so far unsuccessfully.<\/jats:p>","DOI":"10.1128\/iai.00175-22","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T13:00:11Z","timestamp":1654693211000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Targeted Transcriptomic Screen of Pneumococcal Genes Expressed during Murine and Human Infection"],"prefix":"10.1128","volume":"90","author":[{"given":"Alan","family":"Basset","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Wall","sequence":"additional","affiliation":[{"name":"Research Division of Infection, University College London, London, United Kingdom"},{"name":"Francis Crick Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Mitsi","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chloe","family":"Deshusses","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raecliffe","family":"Daly","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherin","family":"Pojar","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Rein\u00e9","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Afonso","family":"Guerra-Assuncao","sequence":"additional","affiliation":[{"name":"Research Division of Infection, University College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Denis","sequence":"additional","affiliation":[{"name":"Malawi Liverpool Wellcome Trust, Blantyre, Malawi"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon P.","family":"Jochems","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Heyderman","sequence":"additional","affiliation":[{"name":"Research Division of Infection, University College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Brown","sequence":"additional","affiliation":[{"name":"Research Division of Infection, University College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Jie","family":"Lu","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela M.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-3398","authenticated-orcid":true,"given":"Richard","family":"Malley","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"177","article-title":"Pneumococcal conjugate vaccines in infants and children under 5 years of age: WHO position paper","volume":"8","author":"WHO","year":"2019","unstructured":"WHO. 2019. Pneumococcal conjugate vaccines in infants and children under 5 years of age: WHO position paper. Wkly Epidemiol Rec 8:177\u2013183.","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2020.02.016"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268820000813"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1774"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/20469047.2019.1623572"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000002489"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.10.046"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62225-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1045"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.08.048"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2018.1532249"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.02.113"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.10.090"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9050396"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2017.1393335"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121383109"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2011.04.002"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-11-46"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1385"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep25533"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4870-4873.2001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.09.076"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.03.071"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1054-5"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky750"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-71910-1"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gdata.2017.02.013"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10101322"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2010428117"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2008.09.008"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00715-20"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00631-10"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00425-08"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002989"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.09.031"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000002454"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.603623"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.03.049"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00175-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00175-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T13:03:24Z","timestamp":1658408604000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.00175-22"}},"subtitle":[],"editor":[{"given":"Liise-anne","family":"Pirofski","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,7,21]]},"references-count":38,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7,21]]}},"alternative-id":["10.1128\/iai.00175-22"],"URL":"https:\/\/doi.org\/10.1128\/iai.00175-22","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"type":"print","value":"0019-9567"},{"type":"electronic","value":"1098-5522"}],"subject":[],"published":{"date-parts":[[2022,7,21]]},"assertion":[{"value":"2022-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00175-22"}}