{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:18:41Z","timestamp":1791425921427,"version":"4.3.3"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The surface proteins of<jats:italic>Ehrlichia chaffeensis<\/jats:italic>provide an important interface for pathogen-host interactions. To investigate the surface proteins of<jats:italic>E. chaffeensis<\/jats:italic>, membrane-impermeable, cleavable Sulfo-NHS-SS-Biotin was used to label intact bacteria. The biotinylated bacterial surface proteins were isolated by streptavidin-agarose affinity purification. The affinity-captured proteins were separated by electrophoresis, and five relatively abundant protein bands containing immunoreactive proteins were subjected to capillary-liquid chromatography-nanospray tandem mass spectrometry analysis. Nineteen out of 22 OMP-1\/P28 family proteins, including P28 (which previously was shown to be surface exposed), were detected in<jats:italic>E. chaffeensis<\/jats:italic>cultured in human monocytic leukemia THP-1 cells. For the first time, with the exception of P28 and P28-1, 17 OMP-1\/P28 family proteins were demonstrated to be expressed at the protein level. The surface exposure of OMP-1A and OMP-1N was verified by immunofluorescence microscopy. OMP-1B was undetectable either by surface biotinylation or by Western blotting of the whole bacterial lysate, suggesting that it is not expressed by<jats:italic>E. chaffeensis<\/jats:italic>cultured in THP-1 cells. Additional<jats:italic>E. chaffeensis<\/jats:italic>surface proteins detected were OMP85, hypothetical protein ECH_0525 (here named Esp73), immunodominant surface protein gp47, and 11 other proteins. The identification of<jats:italic>E. chaffeensis<\/jats:italic>surface-exposed proteins provides novel insights into the<jats:italic>E. chaffeensis<\/jats:italic>surface and lays the foundation for rational studies on pathogen-host interactions and vaccine development.<\/jats:p>","DOI":"10.1128\/iai.00188-07","type":"journal-article","created":{"date-parts":[[2007,5,22]],"date-time":"2007-05-22T16:39:04Z","timestamp":1179851944000},"page":"3833-3841","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Surface-Exposed Proteins of<i>Ehrlichia chaffeensis<\/i>"],"prefix":"10.1128","volume":"75","author":[{"given":"Yan","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Rikihisa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.11.6702"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh417"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.4.1455-1461.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.14.4782-4791.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5406-5413.1998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.6.731-735.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.50.1.TM0500010052"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4772-4776.2002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.4853-4863.2005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.12.2741-2745.1991"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.73.400"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.711-720.2006"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/32.3.368"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.1252-1257.1998"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.3.505-513.1998"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500253"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00720.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-1-87"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020021"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01548-06"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2643-2648.1996"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.3.1419"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.3.1855"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00322.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1681-1686.1997"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1824-1831.2002"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8109-8118.2005"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2516-2524.2003"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2700-2703.2002"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00643.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2083-2091.2001"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.1.132-139.1998"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2496-2502.1997"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.11.3666-3669.1989"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3945825"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.4049\/jimmunol.133.2.1010","volume":"133","year":"1984","unstructured":"Palmer, G. 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