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Epidemiological data suggest that the GI tract serves as a reservoir from which\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      can spread and cause invasive disease or transmit to another host. DksA is a conserved stringent response transcriptional regulator that was identified in an\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      transposon mutagenesis screen as an important\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      gut determinant. However, its role in\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      pathogenesis and gut colonization remain uncharacterized. Here, we demonstrate that DksA is required for survival against membrane-targeting antibiotics, consistent with a role in cell envelope stress tolerance. In addition, DksA positively influences capsule biosynthesis gene expression and hypermucoviscosity, and is essential for robust biofilm formation. Using a murine model, we establish that DksA functions as a determinant of GI colonization, despite disruption of the resident gut microbiota. Furthermore, we demonstrate that DksA is important for environmental survival and transmission by regulating RpoS, thereby providing a mechanistic link between the stringent stress response, environmental survival, and subsequent transmission. Together, these findings establish DksA as a central integrator of the stringent response, coordinating membrane stress resistance, virulence traits, and gastrointestinal colonization in\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00194-26","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:47:09Z","timestamp":1788533229000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["DksA-dependent stringent stress response drives virulence and gastrointestinal persistence of\n                    <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1128","volume":"94","author":[{"given":"Md. 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